illustra™ triplePrep kit är utformad för att ge snabb isolering och rening av höga utbyten av genomiskt DNA (gDNA), total mängd RNA och total mängd denaturerade proteiner från odelade djurvävnader och däggdjursceller. Det effektiva arbetsflödet minskar det totala antalet steg, så att alla tre analyterna kan beredas på mindre än 1 timme.

• High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
• Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
• Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
• Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests

The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μl sample. Input sample volumes can vary from 50 μl to 1 ml. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.