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illustra™ triplePrep, kit
illustra™ triplePrep, kit
Katalog # 28-9425-44
Leverantör:  Cytiva
CAS-nummer:  
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illustra™ triplePrep, kit
Katalog # 28-9425-44
Leverantör:  Cytiva
CAS-nummer:  

Specifikationer

  • Förp.:
    50 Tests
  • Method / Format:
    Precipitation
    Column (silica-based)
  • Prep size:
    Mini
  • Refine target molecule:
    Total RNA
    Total DNA
    Total protein
  • Sample type:
    Cultured cells
    Human/animal cells and tissues
  • Target molecule:
    DNA
    RNA
    Protein
    Co-purification of RNA, DNA and protein
  • Beskrivning:
    illustra™ triplePrep kit, 50 beredningar
  • Benämning:
    illustra™ triplePrep Kit

Specifikationer

Om denna produkt

illustra™ triplePrep kit är utformad för att ge snabb isolering och rening av höga utbyten av genomiskt DNA (gDNA), total mängd RNA och total mängd denaturerade proteiner från odelade djurvävnader och däggdjursceller. Det effektiva arbetsflödet minskar det totala antalet steg, så att alla tre analyterna kan beredas på mindre än 1 timme.

  • High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
  • Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
  • Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
  • Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests

The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μl sample. Input sample volumes can vary from 50 μl to 1 ml. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.

Ordering information: Kit contains lysis buffer type 15 (75 ml), wash buffer type 6 (12 ml), elution buffer type 5 (12 ml), elution buffer type 9 (7,5 ml), protein precipitation buffer type 1 (2×17,5 m)l, one vial DNase I, DNase reaction buffer type 1 (1,4 ml), illustra Mini columns (50X DNA), illustra Mini columns (50X RNA), collection tubes (200) and an instruction booklet.
Delivery: Kit contains illustra blood genomicPrep spin columns prepacked with a novel silica membrane, lyophilised proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
Caution: Yields and purities of gDNA, total RNA, and total denatured proteins can vary with user and depend on the nature and condition of the input sample.