Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

• Ideal for confirmatory Western blotting applications using chemiluminescence detection
• At least 10-fold more sensitive than colorimetric assays
• Detectable on Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500.

Discover our Western Blotting: tips and troubleshooting eLearning course. Learn approaches to ensure high-quality western blotting courses and receive a certificate upon course completion.

High sensitivity chemiluminescence detection on Western blotting
Amersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection, which is suitable for all routine Western blotting. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications.

Reliable chemiluminescence detection
In most contemporary ECL systems a luminol peroxide detection reagent is added to the membrane and reacts with the horseradish peroxidase enzyme (HRP) conjugated to the secondary antibody. HRP catalyzes the oxidation of luminol in a multistep reaction and is accompanied by the emission of low-intensity light at 428 nm, which can be measured with light-sensitive X-ray film or with a CCD imager.