Specifications
- Förp.:1 KIT
- Analystyp:Direct
- Target protein:cGMP
- Beskrivning:Direct cGMP ELISA kit
- Seq:02
- Känslighet:(non-acetylated) 0,604 pmol/ml; (acetylated) 0,025 pmol/ml (range (non-acetylated) 0,8 - 500 pmol/ml; (acetylated) 0,08 - 50 pmol/ml)
Specifications
About this item
Most sensitive (0,025 pmol/ml) colorimetric kit for intracellular cGMP quantification.
For the quantitative determination of cGMP in cell lysates and tissue from any species. Cited sample types include cerebral spinal fluid and microdialysate.
This is a colorimetric competitive immunoassay kit for the quantitation of intracellular cGMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.