- Antikroppstyp:Primär
- Antikroppsnamn:Cyclin-dependent Kinase 1
- Antigensymbol:CDK1
- Klonalitet:Polyclonal
- Konjugation:HRP (Horseradish Peroxidase)
- Värd:Rabbit
- ImmunKemi:Yes
- Isotyp:IgG
- Reaktivitet:Human,Rat,Mouse
- Western blot:Yes
- Korsad adsorption:No
- Form:liquid
- Gen-ID:983
- Antigen synonymer:p34 protein kinase|cell division control protein 2 homolog|P34CDC2|Cell Division Cycle 2|CDC2|CDC28A|G1 to S and G2 to M|cell cycle controller CDC2|cell division protein kinase 1
- Modifiering:phospho-Tyr15
- Storage buffer:Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% Gentamicin. Store at 4°C for 12 months.
- Förvaringstemperatur:Store at 4°C for 12 months
- Koncentration:1 μg/μl
- Shipping temperature:4°C
- Immunogen:5-25/297
- Rening:Purified by Protein A
- Förp.:100 µl
Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair.
Recommended Dilutions: Western Blot: 1:100-1000; IHC-P: 1:100-500
Type: Primary
Antigen: CDK1
Clonality: Polyclonal
Clone: 0
Conjugation: HRP (Horseradish Peroxidase)
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: