Specifications
- Antikroppstyp:Primär
- Antikroppsnamn:Tropomyosin-related kinase receptor
- Antigensymbol:TrkA
- Klonalitet:Monoclonal
- Klon:BS292
- Konjugation:Unconjugated
- Flödescytometri:Yes
- Värd:Mouse
- ImmunKemi:Yes
- ImmunoFluorescens:Yes
- Isotyp:IgG3 kappa
- Reaktivitet:Human
- Western blot:Yes
- Epitop:A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
Sequence - Mått:Protein G purified IgG.
- Form:Lyophilized
- Gen-ID:P04629; NTRK1_HUMAN
- Antigen synonymer:High affinity nerve growth factor receptor|Trk-A|TRK1 transforming tyrosine kinase protein|Neurotrophic tyrosine kinase receptor type 1|NTRK1|TRK|p140-TrkA
- Storage buffer:PBS, pH 7.4, with 3% Trehalose, without preservatives
- Sequence:C-HGPDAKLLAGGE
- Förvaringstemperatur:After reconstitution divide in to aliquots and store at −20 °C for a higher stability. Storage at 4 °C with an appropriate antibacterial agent.
- Shipping temperature:Room (ambient) temperature.
- Immunogen:A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
Sequence - Rening:Protein G purified.
- Storlek:100 ug
- Förp.:100 µG
Specifications
About this item
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).
WB: Western blotting: 1-3 µg/mL, antibody is suitable for reduced (with DTT or ?-mercaptoethanol) and non-reduced samples. Denatured but not reduced samples provides cleaner blot signals as demonstrated in our photographs.
Immunocytochemistry: 1-2 µg/mL. Antibody works on 4% formaldehyde-fixed cells. Note that cells require a permeabilization step, because the antibody detects a cytoplasmic epitope of TrkA.
Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: TrkA
Clonality: Monoclonal
Clone: BS292
Conjugation: Unconjugated
Epitope: A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
Sequence
Host: Mouse
Isotype: IgG3, k-light chain
Reactivity: Human