Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.
- One-tube master mix helps reduce pipetting steps and enhanced consistency in RT-qPCR results
- Increased RT efficiency across a wide range of input RNA amounts and gene targets
- High thermostability to allow RT reactions at 50 to 65 °C temperature range
The master mix contains Maxima™ H Minus Reverse Transcriptase (RT) and RiboLock™ RNase Inhibitor. Maxima™ H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity. The recombinant RiboLock™ RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55 °C. The master mix also contains reaction buffer, dNTPs, oligo (dT)18, and random hexamer primers. A separate tube of No-RT control mix is also provided.