The HDAC/SIRT chemiluminescent drug discovery kit is a complete assay system designed to measure histone deacetylase (HDAC) and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes.

• High specificity assay eliminates false positives or negatives
• Detects without any of the side effects
• Superior signal-to-noise ratio with no interference from cell extract detergents
• Delivers consistent results from a validated system

The HDAC Chemiluminescent activity assay is based on the CHEMILUM DE LYS® substrate and developer combination. The CHEMILUM DE LYS® system (chemiluminescent histone deAcetylase lysyl substrate/developer) is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases.

The assay procedure has three steps. Firstly, the CHEMILUM DE LYS® substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitises the substrate so that, in the second step, treatment with the CHEMILUM DE LYS® Developer followed by Enhancer produces light. The reaction is luciferase-free.