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HDAC/SIRT chemiluminescent drug discovery kit, CHEMILUM DE LYS®
HDAC/SIRT chemiluminescent drug discovery kit, CHEMILUM DE LYS®
Catalog # ENZOBMLAK5320001
Supplier:  ENZO LIFE SCIENCES
CAS Number:  
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HDAC/SIRT chemiluminescent drug discovery kit, CHEMILUM DE LYS®
Catalog # ENZOBMLAK5320001
Supplier:  ENZO LIFE SCIENCES
CAS Number:  

Specifications

  • Förp.:
    1 KIT
  • Beskrivning:
    CHEMILUM DE LYS® HDAC/SIRT chemiluminescent drug discovery kit
  • Seq:
    02

Specifications

About this item

The HDAC/SIRT chemiluminescent drug discovery kit is a complete assay system designed to measure histone deacetylase (HDAC) and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes.

  • High specificity assay eliminates false positives or negatives
  • Detects without any of the side effects
  • Superior signal-to-noise ratio with no interference from cell extract detergents
  • Delivers consistent results from a validated system

The HDAC Chemiluminescent activity assay is based on the CHEMILUM DE LYS® substrate and developer combination. The CHEMILUM DE LYS® system (chemiluminescent histone deAcetylase lysyl substrate/developer) is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases.

The assay procedure has three steps. Firstly, the CHEMILUM DE LYS® substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitises the substrate so that, in the second step, treatment with the CHEMILUM DE LYS® Developer followed by Enhancer produces light. The reaction is luciferase-free.

Delivery: Kit contains Nuclear extract from HeLa cells (human cervical cancer cell line), CHEMILUM DE LYS® substrate, CHEMILUM DE LYS® developer concentrate (20X), trichostatin A (HDAC inhibitor), NAD (sirtuin substrate), nicotinamide (sirtuin inhibitor), HDAC assay buffer, developer buffer, CHEMILUM DE LYS® enhancer part A, CHEMILUM DE LYS® enhancer part B and a half volume white microplate.