Expression vectors for PCR-based generation of linear templates.

RTS pIX3.0 Vector: Cloning Vector for RTS Linear Templates
Multiple cloning site compatible with Linear Templates generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit
High copy origin of replication
Upscaling cell-free protein synthesis to CECF (continous exchange cell-free) scale after expression screening using Linear Templates
Free of interfering regulatory elements for transcription and translation, which are solely contributed by Linear Templates
Compatible with RTS 100 E. coli HY kit and RTS Insect Kit series

RTS pIX4.0 Insect Vector: Expression vector for efficient synthesis of proteins using the RTS 100 Insect Membrane Kits and RTS 100 Insect Disulfide Kits
Efficient initiation of translation through a T7 promoter element combined with an optimized 3' UTR, a T7 terminator, and an optimally positioned linearisation site
Multiple cloning site and translational stop codons in all reading frames for convenient preparation of expression constructs
Optimised 3' UTR combined with T7 terminator for generation of stabilized RNA protected from degradation by exonucleolytic nucleases
Site for plasmid linearisation consisting of multiple restriction sites for blunt end linearisation for effective in vitro transcription

RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set: Vectors for preparative scale expression of 6xHis-tagged proteins
Stable circular templates ensure high yields in preparative scale expression reactions
For construction of C- and N-terminal 6xHis-tagged proteins
Optimal spacing of all regulatory elements
For use with RTS 100, RTS 500, and RTS 9000 E. coli HY reactions

RTS pIVEX Wheat Germ His6-tag Vector Set: Expression vector for preparative scale expression in eukaryotic wheat germ lysates
Stable circular templates ensure high yields in preparative scale expression reactions
For contruction of C- and N-terminal 6xHis-tagged proteins
Easily detect and purify expressed proteins via 6xHis-tag