The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.
The Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay is supplied with the Reducing Agent Compatibility Agent (RACA) that modifies reducing agents to limit their effect on the reduction of the assay’s copper ions, preventing inhibition of the assay. The use of RACA allows for samples containing up to 5 mM DTT, 10 mM TCEP or 35 mM ß-mercaptoethanol.
The Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay is suitable for quantifying protein solutions in 1 ml assays or in microwells. The kit is suitable for up to 250×1 ml assays.