The AxyPrep™ Mag Tissue gDNA Kit utilises a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.

The protocol comprises three easy steps to effectively remove cellular contaminants from gDNA. The first step involves lysing the tissue sample with a lysis buffer containing DTT and Proteinase K. Next, the DNA is bound to the paramagnetic beads and separated from the solution. Cellular contaminants, such as proteins and debris are then washed away and the DNA is easily eluted off the beads to be used in downstream applications. The AxyPrep™ Mag Tissue-Blood genomic DNA is adaptable to various commercially available automation platforms and may be performed in both 96-well and single tube formats.