Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure
Used in Western blotting protocols.
- Contaminant testing:
- DNase (endo): Passes test
- DNase (exo): Passes test
- RNase: Passes test
- Protease: Passes test
20X TBS (Tris Buffered Saline) solution consisting of 500 mM Tris, 60 mM KCl and 2,8 M NaCl in high purity dH₂O, pH 7,4. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles. Dilute to 1X with high purity water for a solution consisting of 25 mM Tris, pH 7,4, 3,0 mM KCl and 140 mM NaCl. With pH (25 °C, 20X): 7,4 ±0,1.