Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.
- Eliminates the need for glass bead lysis
- Supplied as a kit containing Zymolyase® and reagents needed for protein extraction
- Suitable for processing 100×50 µl yeast cell pellet preparations
- Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis
A ready to use Zymolyase® preparation is also provided. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Yeast PE LB™. Yeast PE LB™ has been tested on several widely used yeast strains.
The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells.