Supplier: ENZO LIFE SCIENCES

Clostridium difficile toxoid A from Clostridium difficile, strain VPI10463 is a high potency toxin applicable to ELISA, antibody capture, cytotoxicity studies, immunogen and LINE assay.

Supplier: THERMO FISHER DIAGNOSTICS

Directly detect antigens present in human cerebrospinal fluid (CSF), serum, urine, or blood cultures using Thermo Scientific™ Wellcogen™ Bacterial Antigen Rapid Latex Agglutination Test.

Supplier: THERMO FISHER DIAGNOSTICS

Wellcolex® Rapid Latex Agglutination test kits are designed for rapid and accurate identification of enteric bacteria, such as Salmonella, Shigella and E.coli. With its high positive and negative predictive values (PPV >98,7%) and results in less than three minutes, laboratories can use this rapid screening test to eliminate Salmonella-negative samples from further testing, reducing the number of samples requiring confirmatory testing.

Supplier: ENZO LIFE SCIENCES

Clostridium difficile toxin A from Clostridium difficile, strain VPI10463 is a high potency toxin applicable to ELISA, antibody capture, cytotoxicity studies and LINE assay.

Supplier: ENZO LIFE SCIENCES

Clostridium difficile toxoid A from Clostridium difficile, strain VPI10463 is a high potency toxin applicable to ELISA, antibody capture, cytotoxicity studies, immunogen and LINE assay.

Supplier: Merck

EHEC is capable of inducing life threatening illnesses, particularly in people with immune deficiency, young children and the elderly. Although the most common cause of EHEC is E. coli O157, other serotypes, such as O26, O45, O103, O111, O121 and O145, are also relatively frequent causes of infection. Typical symptoms are severe abdominal pain and diarrhoea, which is initially watery but often becomes bloody. E. coli are gram-negative, non sporulating, facultative anaerobic bacilli. Amongst the pathogenic E. coli, Verotoxin-forming strains (STEC or VTEC) have gained importance in recent years. The group of enterohaemorrhagic E. coli (EHEC) with its highly pathogenic serovar O157:H7 strain is particularly interesting in this respect. The main sources of infection are contaminated, raw or insufficiently heated foods of animal origin, such as meat and dairy products. The reservoir for EHEC is the gut of ruminants. Microorganisms can enter food during the processing of meat and dairy products if hygienic conditions are inadequate. The drastic increase in the incidence of food contamination caused by E. coli O157 demands reliable and rapid methods of detection. Innovative, rapid tests make screening for pathogenic organisms easier. Complete test sets for the detection of pathogens and toxins. The one-step format reduces the possibility of errors during handling. In addition to tests for the most common cause of infection, E. coli O157, there exits a 24-H screening protocol for VTEC, including E. coli O104:H4, based on Rapidcult™ E. coli enrichment combined with detection using Duopath® Verotoxins.

Supplier: THERMO FISHER DIAGNOSTICS

Antigen-Antibody Tests, Samonella Typhi O (Group D) (9,12)

Supplier: Merck

Most individuals infected with Salmonella develop diarrhea, fever, and abdominal cramps 12 to 72 H after infection. The illness usually lasts 4 to 7 days, and the majority of patients recover without treatment. However, in some cases, the diarrhea may be so severe that hospitalisation is required. Salmonella is a genus of rod-shaped, Gram-negative, non spore-forming, predominantly motile bacteria, belonging to the Enterobacteriaceae family. Salmonella spp. are one of the most common causes of food poisoning worldwide and have been isolated from most types of raw food (e.g. meats, eggs and plant products). Their high resistance to drying combined with a very high heat resistance once dried makes Salmonella a potential problem in most foods, particularly in dry and semi-dry products. Food legislation in many countries includes limits on Salmonella – typically absence in 25 g of food. Traditional microbiological methods for detection of Salmonella in food and animal feed require a total of up to five days to obtain a simple yes/no result. Innovative, rapid tests make screening for pathogenic organisms easier. The one-step format reduces the possibility of errors during handling.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of hepatitis viruses.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of human herpes viruses.

Supplier: BIOPREMIER

These kits allow the detection of allergic substances. Ideal for controlling and monitoring the various steps of food production.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of gastrointestinal pathogens.

Supplier: BIOPREMIER

SUPREME Real time detection uses real-time PCR for the detection of DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target.

Supplier: Genscript

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit is designed to be a quantitative In vitro end-point endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

Supplier: MACHEREY-NAGEL

These products are used in testing the resistance of pathogens to antibiotics. For this test the filter paper sections can be impregnated with the antibiotic to be tested and placed on the inoculated nutrient medium. Depending on the effectiveness, a smaller or larger zone of inhibition is formed.

Supplier: Genscript

ToxinSensor™ Gel Clot Endotoxin Assay Kit is intended as an in vitro end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices. The limulus amebocyte lysate (LAL) test is a qualitative test for Gram-negative bacterial endotoxin.

Supplier: Sartorius

The Microsart® ATMP Mycoplasma detection kit is especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

Supplier: ROSCO

Antibiotic Tests, Total ESBL + Ampc Confirm Kit

Supplier: ROSCO

Antibiotic Tests, One cartridge single dispenser

Supplier: ROSCO

Neo-Sensitabs are tablets manufactured by a process using crystalline antimicrobials. Extensive range - over 125 different antimicrobial agents and combination tablets are available. Detection of mechanisms of resistance including MRSA, ESBL, AmpC Inducers and Inducible Clindamycin resistance. Logical 5-figure alpha-numeric code, easy and safe identification of the individual tablet, optically readable by automatic reader systems. Neo-Sensitabs are standardized to CLSI recommendations and adjusted to other national guidelines.

Supplier: HYSERVE

To detect allergen in highly processed foods by special extraction solution and unique antibody. This lateral-flow test is a high-performance qualitative assay.

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

Supplier: Sartorius

The Microsart® RESEARCH Mycoplasma real-time PCR kit is especially designed for fast and reliable Mycoplasma contamination control in cell cultures, cell culture supernatants and media most applicable in research and development, e.g. biotech and biopharmaceutical research and development, universities and governmental research groups or for in-process control during biopharmaceutical production processes.

Supplier: PRIMERDESIGN

Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. Although more than 100 different human papillomavirus (HPV) types have been characterised HPV 6, HPV 11, HPV-16 and HPV 18 are the most clinically relevant.

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of an animal species in meat and meat products. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target organism. According to European Commission Directive 2002/86/EC, food ingredients have to be declared. In the case of meat and meat products, the animal species has to be disclosed on the label. Moreover, the species autenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue (Ballin, 2010; Dooley et al., 2004). Several methods are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR-based techniques still allow the identification of DNA from the different species present in a sample (Lockley & Bardsley, 2000). Real-Time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity (Dooley et al., 2004).

Supplier: BIOPREMIER

Gluten detection test kit by using qPCR. Celiac disease is an autoimmune disorder associated with an intolerance to gluten proteins mainly present in wheat, barley, and rye.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. It also includes a test for Shigella. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Vibrio alginolyticus causes eye, ear and wound infections. It can be tested using this Real-Time PCR test. The analysis can be done in water and food samples. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and you avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Listeria monocytogenes in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838.Test principle. The test is dedicated to detect DNA of Listeria monocytogenes in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20 μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Salmonella in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838. In combination with an appropriate pre-enrichment and DNA preparation the test complies with the official method of the German food and feed law §64. ASU L00.00-98. Test principle: The test is dedicated to detect DNA of Salmonella in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the ISO standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.