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Supplier: Merck Millipore (Novagen)

Molekylviktsmarkörer för rutinanvändning inom plasmidkloning, Perfect DNA™ Markers med lätt ihågkomna storlekar för analys på agarosgel. Markörerna har enhetliga bandintensiteter med undantag för de indikerade referensbanden, för enkel identifiering av band.

Supplier: LONZA

DNA marker is for rapid size estimation of PCR products and restriction fragments. DNA can be visualised by ethidium bromide staining or kinased with radiolabelled ³²P for detection by autoradiography.

Supplier: Merck Millipore (Novagen)

Perfect DNA™ Ladders är DNA-stegar med behändiga, lätt ihågkomna storlekar för analys på agarosgel. Markörerna har enhetliga bandintensiteter och täcker en lång rad DNA-storlekar.

Supplier: LONZA

QuantLadders are prepared from five highly purified DNA fragments that provide visual quantitation of DNA within ±1% accuracy. The DNA QuantLadder provides each DNA fragment in perfect relative mass equivalence at 100 ng, 70 ng, 50 ng, 20 ng and 10 ng. The reverse QuantLadder simply reverses the mass equivalence order of the QuantLadder.

Supplier: PanReac AppliChem

This DNA ladder is ideal for determining the size of double-stranded DNA ranging from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.

Supplier: Biotium

A visible three- colour protein marker for SDS-PAGE or western blotting, with 12 bands ranging from 8 to 245 kDa.

Supplier: Thermo Fisher Scientific

This wide range molecular weight marker contains 60 ng/μl of 21 fragments from 100 bp to 12000 bp. The bands appear in 100 bp increments from 100 to 1000 bp and in 1000 bp increments from 1 to 12 kb. Bands at 1 kb and 5 kb stain brighter to aid identification.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

Supplier: LONZA

ProSieve™ Color protein marker have been labelled with three different fluorescent dyes. These markers are supplied as a lyophilised solid containing the buffer salts and detergent found in the typical Laemmli buffer system. It contains nine proteins with approximate masses of 10, 15, 20, 25, 40, 50, 80, 125, and 190 kDa.

Supplier: Biotium

A visible three-colour protein marker for SDS-PAGE or western blotting, with 10 bands ranging from 10 to 180 kDa.

Supplier: PanReac AppliChem

The main applications for prestained protein markers are the monitoring of protein migration during SDS-PAGE, verification of Western transfer efficiency on membranes, and estimation of protein sizes on SDS-polyacrylamide gels and Western blots.

Supplier: LONZA

Thse DNA ladders provides rapid and easy size determination of the DNA samples. Each band has an ideal 50 % G-C content for precise sizing and sharp resolution. It is free from contaminating plasmid DNA with minimal background.

Supplier: Merck Millipore (Oncogene)

This Western-Re-Probe™ reagent is validated for use in immunoblotting.

Supplier: Rockland Immunochemicals

FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Supplier: Thermo Fisher Scientific

SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Supplier: G-Biosciences

Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

Supplier: Rockland Immunochemicals

TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Supplier: Thermo Fisher Scientific

The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Supplier: G-Biosciences

Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Supplier: Thermo Fisher Scientific

Saponin permeating solution, is used as a cell permeabilising agent.

Supplier: Thermo Fisher Scientific

QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

Supplier: Thermo Fisher Scientific

For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

Supplier: G-Biosciences

Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, E. coli, Rhizobium and green algae.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Supplier: PanReac AppliChem

The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.

Supplier: Rockland Immunochemicals

These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Supplier: Cytiva

Med Rainbow™ molekylviktsmarkörer går det snabbare och enklare att identifiera proteiner på SDS-polyakrylamidgel. De laddningsklara markörerna ger skarpare och mer intensiva band på gel och blot och har förbättrade bandavstånd för mer exakt angivelse av molekylvikter. Markörernas klara och distinkta färger gör det enklare att bekräfta överföringen till blotting-membran och riktning.

Supplier: Cytiva

pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.