Du sökte efter: Elektroforesreagens

Supplier: Thermo Fisher Scientific

Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

Supplier: AAT BIOQUEST

Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

Supplier: Thermo Fisher Scientific

SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

Supplier: Thermo Fisher Scientific

The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Supplier: Thermo Fisher Scientific

Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

Supplier: Thermo Fisher Scientific

DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. They are commonly used in the studies of plasmid DNA and PCR products.

Supplier: Thermo Fisher Scientific

From 250 to 5000 base pairs: 5 bands from 125 to 1000 bp; four bands from 1500 to 5000 bp. Recommended load volume 20 µl.

Supplier: Merck Millipore (Novagen)

PCR Markers innehåller en blandning av åtta definierade DNA-fragment från 50 till 2000 bp med praktiska storleksintervaller för att karaktärisera små DNA-produkter. Markörerna levereras i en gelladdningsbuffert som innehåller två spårningsfärger som inte stör UV-visualisering av band färgade med etidiumbromid. En separat vial med 6X laddningsbuffert är inkluderad. Varje vial med markör räcker till 50 körningar på TBE eller TAE agaros färgad med etidiumbromid. Den rekommenderade mängden för laddning (5 µl) ger band med en jämn intensitet som är klara, skarpa och lätta att fotografera.

Supplier: LONZA

DNA marker is for rapid size estimation of PCR products and restriction fragments. DNA can be visualised by ethidium bromide staining or kinased with radiolabelled ³²P for detection by autoradiography.

Supplier: G-Biosciences

DNAmark™ 1 kb Plus ladder contains 15 DNA bands ranging from 100 bp to 10 kb. The 500 bp and 3000 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 3000 bp are 40 ng. The 500 bp and 3000 bp fragment are 100 ng.

Supplier: Merck Millipore (Novagen)

Molekylviktsmarkörer för rutinanvändning inom plasmidkloning, Perfect DNA™ Markers med lätt ihågkomna storlekar för analys på agarosgel. Markörerna har enhetliga bandintensiteter med undantag för de indikerade referensbanden, för enkel identifiering av band.

Supplier: G-Biosciences

DNAmark™ 100 bp Plus ladder consists of 14 DNA fragments ranging in size from 100 to 3000 base pairs (bp). The 500 bp and 1200 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 1200 bp are 40 ng. The 500 bp and 1200 bp fragment are 100 ng.

Supplier: G-Biosciences

DNAmark™ 100 bp ladder consists of 11 fragments ranging from 100 to 1500 base pairs (bp). The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp are 40 ng. The 500 bp fragment is 100 ng.

Supplier: G-Biosciences

DNAmark™ 500 bp ladder consists of 10 DNA fragments ranging from 500 bp to 5000 bp. The 2000 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 2000 bp are 40 ng. The 2000 bp fragment is 100 ng.

Supplier: LONZA

QuantLadders are prepared from five highly purified DNA fragments that provide visual quantitation of DNA within ±1% accuracy. The DNA QuantLadder provides each DNA fragment in perfect relative mass equivalence at 100 ng, 70 ng, 50 ng, 20 ng and 10 ng. The reverse QuantLadder simply reverses the mass equivalence order of the QuantLadder.

Supplier: Merck Millipore (Novagen)

Perfect DNA™ Ladders är DNA-stegar med behändiga, lätt ihågkomna storlekar för analys på agarosgel. Markörerna har enhetliga bandintensiteter och täcker en lång rad DNA-storlekar.

Supplier: PanReac AppliChem

This DNA ladder is ideal for determining the size of double-stranded DNA ranging from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.

Supplier: Thermo Fisher Scientific

SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.

Supplier: Thermo Fisher Scientific

LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

Supplier: Thermo Fisher Scientific

Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.

Supplier: Thermo Fisher Scientific

Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Supplier: Thermo Fisher Scientific

SuperSignal® ELISA Femto-substrat med maximal känslighet använder SuperSignal®-systemet tillsammans med en förbättringssammansättning för överlägsen proteindetektion och lågnivå linjäritet vid ELISA-tillämpningar. Idealiska för automatiserade screeningtillämpningar med hög genomströmning.

Supplier: Thermo Fisher Scientific

The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Supplier: Thermo Fisher Scientific

These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

Supplier: Thermo Fisher Scientific

OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.

Supplier: Thermo Fisher Scientific

Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.

Supplier: Thermo Fisher Scientific

This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

Supplier: Thermo Fisher Scientific

The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.

Supplier: Thermo Fisher Scientific

QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.