Proteinreningsreagens
Reagenser för proteinrening används för att isolera ett eller flera proteiner från en komplex blandning, vanligtvis celler, vävnader eller hela organismer. Denna process är avgörande för funktions- och strukturkarakterisering samt för att identifiera interaktioner hos det intressanta proteinet. Under de många stegen hjälper proteinreningsreagenser till med celllys, separation av de lösliga proteinkomponenterna från cellrester och slutligen separation av målproteinet från produkt- och processrelaterade föroreningar.
Pierce™ Recombinant Protein G, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.
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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
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Recombinant protein A/G, peroxidase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
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Recombinant protein A/G, alkaline phosphatase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
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Immobilised TCEP disulphide reducing gel, Pierce™
Supplier: Thermo Fisher Scientific
Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.
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PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.
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Protein stabilising cocktail, Pierce™
Supplier: Thermo Fisher Scientific
The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.
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Inclusion body solubilisation reagent, Pierce™
Supplier: Thermo Fisher Scientific
Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
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Recombinant protein G, peroxidase conjugated, Calbiochem®
Supplier: Merck Millipore (Oncogene)
Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.
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HCCA matrix
Supplier: BRUKER DALTRONIK
Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.
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BTSNM support materials, beads, 0,4 g with buffer
Supplier: G-Biosciences
Beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.
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Recombinant streptavidin
Supplier: G-Biosciences
Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.
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Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound
Supplier: Thermo Fisher Scientific
Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.
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Coupling catalyst, GlycoLink™
Supplier: Thermo Fisher Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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Mass spectrometry reagents, MALDI matrices, single-use
Supplier: Thermo Fisher Scientific
MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.
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Pegylation reagents, methyl-PEG-amine compounds
Supplier: Thermo Fisher Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.



