Buffertar för cellodling
Vävnadskulturbuffertar ger en energikälla, upprätthåller korrekt pH-nivå och håller ett idealiskt osmotiskt tryck. Används främst inom biomolekylär forskning, där de torra och flytande substanserna har en balanserad sammansättning av oorganiska salter. Alternativ med lägre koncentration tillåter utjämning med luft i ett slutet system, medan alternativ med högre salthalt håller gasfaser i jämvikt. Celltillväxt är optimal när de odlas i ett näringsmedium som har buffrats.
VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology
Supplier: VWR Chemicals
137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer
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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology
Supplier: VWR Chemicals
Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)
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VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)
Supplier: VWR Chemicals
Dulbecco's formulation without calcium and magnesium.
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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: VWR Chemicals
Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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VWR®, Cell culture salt solutions, Dulbecco's Phosphate Buffered Saline (DPBS)
Supplier: VWR Chemicals
DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.
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Dulbecco's phosphate buffered saline (DPBS), for cell culture, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications, (liquid form).
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VWR® UltraPure, Phosphate buffered saline (PBS), powdered
Supplier: VWR Chemicals
PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.
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VWR®, Phosphate Buffered Saline (PBS), pH 7,4
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.
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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications (powder form).
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HEPES Buffer Solution 1 M, HyClone™
Supplier: HyClone products (Cytiva)
Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.
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Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solutions for cell culture applications.
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Phosphate Buffered Saline
Supplier: THERMO FISHER DIAGNOSTICS
Cell culture buffer, Phosphate buffered saline tablets
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Cell culture salts, salt solutions and buffers
Supplier: BIOWEST
These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.
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Hank's balanced salt solutions (HBSS), Corning®
Supplier: Corning
Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.
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Phosphate-buffered saline (PBS), Corning®
Supplier: Corning
Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate
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Phosphate buffered saline solutions (PBS) without calcium and magnesium, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications.
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