Du sökte efter: Proteinrenings
Proteinreningssystem är avsedda för isolering av ett eller flera proteiner från en komplex blandning. Oavsett om du arbetar med celler, vävnader eller hela organismer renar maskinerna med fördefinierade startpunkter och redigerbara metodmallar. De kompakt utformade proteinreningsapparater är enkelt att använda och har realtidskontroll och visualisering. De kan användas i kylrum för rening av affinitetsmärkta proteiner, antikroppar, omärkta eller nativa proteiner, samt provrengöring.
Protein purification kits, HOOK™
Supplier: G-Biosciences
For the rapid purification of soluble, 6X His or GST tagged proteins.
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Kolonnfyllnadsmaterial, Fractogel® EMD
Supplier: MERCK PRODUCTION CHEMICALS
Fractogel® presents a range of media with 'tentacular' geometries which provide an enhancement of surface topology that give the resins advantageous features for many production processes. The resulting strong binding of biomolecules by a variety of ligands (depending on application and media chosen) and the long lifetime of the material leads to high efficiency and economy of all Fractogel® media. The process media, based on a synthetic methacrylate resin, have high mechanical strength excellent and high chemical stability making the media easy to pack and ‘clean in place'. In addition the base has several advantages over carbohydrate supports as the polymer resin is resistant to microbial degradation and the risk of contamination with endotoxins is greatly reduced.
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Användbara vätskeextraktionsplattor, J.T.Baker®, BAKERBOND®
Supplier: Avantor
J.T.Baker® BAKERBOND® användbara vätskeextraktionsplattor (SLE) är ett idealiskt och snabbare alternativ till traditionella extraktionsmetoder vätska-vätska och medger briljanta återställningar av analyter från biologiska matriser.
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Jacalin for IgA purification
Supplier: G-Biosciences
Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.
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Proteinfällningsplattor, J.T.Baker® BAKERBOND®
Supplier: Avantor
J.T.Baker® BAKERBOND® proteinfällningsplattor medger snabb borttagning av protein från biologiska prover.
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Proteomic protein controls
Supplier: G-Biosciences
Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.
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Immobilised D-galactose for lectin purification
Supplier: G-Biosciences
Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.
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Pep-Extract™
Supplier: G-Biosciences
Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.
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Concanavalin A agarose
Supplier: G-Biosciences
Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.
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Recombinant protein A/G, Pierce™
Supplier: Thermo Fisher Scientific
Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.
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Maleimide-activated protein, NeutrAvidin™ Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.
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HeLa protein digest standard, Pierce™
Supplier: Thermo Fisher Scientific
The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
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Recombinant protein L, peroxidase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
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Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.
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Recombinant protein A, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.
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Recombinant protein A, peroxidase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
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Immobilised TCEP disulphide reducing gel, Pierce™
Supplier: Thermo Fisher Scientific
Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.
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TCEP solution, neutral pH, Bond-Breaker™ Pierce™
Supplier: Thermo Fisher Scientific
Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.
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Pierce™ Recombinant Protein G, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.
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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
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Recombinant protein A/G, peroxidase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
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Recombinant protein L, biotinylated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Biotinylated Recombinant Protein L can be used to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.
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Recombinant protein A/G, alkaline phosphatase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
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Recombinant protein L, Pierce™
Supplier: Thermo Fisher Scientific
Purified (unconjugated) Pierce™ Recombinant Protein L is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of mouse and human antibodies in immunoassays and antibody purification protocols.
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ReadiPrep™ Nickel NTA Magnetic Beads
Supplier: AAT BIOQUEST
ReadiPrep™ Nickel NTA magnetic beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged proteins.
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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds
Supplier: Thermo Fisher Scientific
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.
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Immobilised ficin (agarose resin), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.
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Pierce™ Protein A
Supplier: Thermo Fisher Scientific
Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
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Inclusion body solubilisation reagent, Pierce™
Supplier: Thermo Fisher Scientific
Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
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J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A
Supplier: Avantor
The J.T.Baker® BAKERBOND® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.