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Proteinrenings

Proteinrenings

Proteinreningssystem är avsedda för isolering av ett eller flera proteiner från en komplex blandning. Oavsett om du arbetar med celler, vävnader eller hela organismer renar maskinerna med fördefinierade startpunkter och redigerbara metodmallar. De kompakt utformade proteinreningsapparater är enkelt att använda och har realtidskontroll och visualisering. De kan användas i kylrum för rening av affinitetsmärkta proteiner, antikroppar, omärkta eller nativa proteiner, samt provrengöring.

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J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A

J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A

Supplier: Avantor

The J.T.Baker® BAKERBOND® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

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Användbara vätskeextraktionsplattor, J.T.Baker®, BAKERBOND®

Användbara vätskeextraktionsplattor, J.T.Baker®, BAKERBOND®

Supplier: Avantor

J.T.Baker® BAKERBOND® användbara vätskeextraktionsplattor (SLE) är ett idealiskt och snabbare alternativ till traditionella extraktionsmetoder vätska-vätska och medger briljanta återställningar av analyter från biologiska matriser.

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Ion exchange chromatography media, Ion Exchanger I

Ion exchange chromatography media, Ion Exchanger I

Supplier: Merck

Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.

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Immunoprecipitation kit, Abcam

Supplier: Abcam

Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.

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HRP Conjugate Stabilizer - LifeXtend, Abcam

Supplier: Abcam

HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature.

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GTP-Agarose, Abcam

Supplier: Abcam

GTP-Agarose (ab270533) comprises GTP attached to agarose beads.

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Mouse TCS Antibody Purification Kit, Abcam

Supplier: Abcam

Efficient mouse antibody purification from tissue culture supernatants.

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Affinity purification Streptavidin Resin and buffers

Supplier: G-Biosciences

Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

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BSA Removal Kit (Gold/Europium labelling), Abcam

Supplier: Abcam

Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.

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Proteinfällningsplattor, J.T.Baker® BAKERBOND®

Proteinfällningsplattor, J.T.Baker® BAKERBOND®

Supplier: Avantor

J.T.Baker® BAKERBOND® proteinfällningsplattor medger snabb borttagning av protein från biologiska prover.

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Hydrofoba interaktionskromatografimedia, J.T. Baker®, BAKERBOND®

Hydrofoba interaktionskromatografimedia, J.T. Baker®, BAKERBOND®

Supplier: Avantor

Kiselbaserat medium som används för rening av proteiner på basis av differentierad saltinducerad hydrofobicitet. Mixed mode medger unik selektivitet med sin hydrofoba interaktion och jonbytesfunktion.

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TMB substrate 1X

TMB substrate 1X

Supplier: Abcam

TMB substrate recommended for use with the Matched Antibody Pair kits for sandwich ELISA.

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Anti-HA Affinity Resin - Amintra, Abcam

Supplier: Abcam

Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.

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TCS Antibody Purification Kit (Gold/Europium labelling), Abcam

Supplier: Abcam

TCS Antibody Purification Kit - Nanoparticles (ab270554) is designed for the purification of IgG fractions from tissue culture supernatants.

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Mouse Antibody Purification Kit, Abcam

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

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