You searched for: Transfermembran
En överföringsmembran eller transfermembran använder sig av kapillärkraft för att separera DNA, RNA eller proteinfragment för detektering. Används transfermembranen på korrekt sätt med de geler and buffertar som krävs för blottingstekniken kan man återanvända samma membran för multipla strippnings- reprobing- och hybridiseringscykler. De högbindande membraner finns som skivor, ark, och rullar med olika porstorlekar och laddningar anpassat till alla blottingstekniker i molekylärbiologi och genetisk forskning.
Transfermembran, Biodyne®
Supplier: Cytiva (Formerly Pall Lab)
Biodyne® transfermembran har hög känslighet och lågt bakgrundsbrus för förbättrad detektion och upplösning. De är idealiska för detektion av nukleinsyror.
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Millipore® Immobilon®-NC Hydrophilic Transfer Membrane
Supplier: Merck
Triton-free, mixed cellulose esters transfer membrane.
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Millipore® Immobilon®-FL NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
Hydrophobic PVDF transfer membrane for western blotting using fluorescent, chemifluorescence, chromogenic, or chemiluminescence.
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Millipore® Immobilon®-NC NOW Bundle Hydrophilic Mixed Cellulose Esters Transfer Membrane
Supplier: Merck
Triton-free, mixed cellulose esters transfer membrane, includes 1 dispenser.
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Millipore® Immobilon®-E NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
The first PVDF transfer membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
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Immobilon®-P Blotting Sandwich Transfer Membranes
Supplier: Merck
Immobilon®-P, Immobilon-P blotting sandwich transfer membranes, 7×8,4 cm
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Millipore® Immobilon®-PSQ NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
PVDF transfer membrane hydrophobic for western blotting using chemiluminescence, chromogenic or radioactive detection methods.
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Millipore® Immobilon®-P NOW Bundle Hydrophobic PVDF Transfer Membrane
Supplier: Merck
Hydrophobic immobilon-P PVDF transfer membrane for western blotting using chemiluminescence, chromogenic or radioactive detection methods.
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Millipore® Immobilon®-NC Nitrocellulose Transfer Membranes
Supplier: Merck
Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth
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Millipore® Immobilon® PVDF Transfer Membranes
Supplier: Merck
These Immobilon® PVDF membranes, each optimised for different protein blotting applications.
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Transfer membranes, PVDF, Pierce™
Supplier: Thermo Fisher Scientific
Transfer membranes made of high quality PVDF, which provides high binding capacity for proteins and nucleic acids.
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Pierce™, Biodyne™ A Blotting Membranes, Nylon
Supplier: Thermo Fisher Scientific
Biodyne™ A nylon membranes are sheets of unmodified nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ A nylon membrane is manufactured with a matrix pore size of 0,45 micron. It contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules.
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Transfer membranes, PVDF, Owl™, Immobilon-P™
Supplier: THERMO OWL SCIENTIFIC
Hydrophobic polyvinylidene fluoride transfer membrane has a high signal-to-noise ratio, which allows for the use of mild blocking procedures
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Överföringsmembran, FluoroTrans® PVDF
Supplier: Cytiva (Formerly Pall Lab)
Naturligt hydrofob PVDF, idealisk för en mängd olika tillämpningar inom proteinanalys. FluoroTrans®-mediefamiljen är vita, mikroporösa fastfasstöd som binder proteiner hårt via hydrofoba interaktioner. FluoroTrans®-membran är optimerade för N-terminal-proteinsekvensering. FluoroTrans® W-membran är optimerade för tillämpningar med Western-överföring. FluoroTrans® W-membran möjliggör känslig proteindetektion med låg bakgrund och mycket låg genombränning av protein. Immobiliserade proteiner kan detekteras visuellt med Coomassie®-blått, Amido-svart, Ponceau S och kolloidalt guld.
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Blotting membranes, nitrocellulose
Supplier: Thermo Fisher Scientific
Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.
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Blotting membranes, nylon, Biodyne™ B
Supplier: Thermo Fisher Scientific
Biodyne™ B nylon membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ B nylon membrane is manufactured with a matrix pore size of 0,4 micron. It contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules, like DNA and RNA.
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Blottingmembran, Nytran® SuPerCharge, Whatman™
Supplier: Whatman products (Cytiva)
Nytran® SuPerCharge (SPC) nylonmembran har en mycket hög positiv laddning och en högre nylondensitet per enhetsområde. Finns cirkelformade eller som ark eller rullar för mikroplattor.
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Whatman™ Protran® BA85 & BA83 NC Western blottingmembran, nitrocellulosa
Supplier: Whatman products (Cytiva)
Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pre treatment steps are necessary.
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Blottingmembran, Nytran® N, Whatman™
Supplier: Whatman products (Cytiva)
Nytran® Nylon-membranet (Nytran® N) är idealiskt för tillämpningar som kräver en lägre laddning. Det är utformat för Southern- och Northern-blotteknik samt för lyft av kolonier och plack och dot- eller slot-blottar. Nytran® N är kompatibelt med isotopiska detektionsmetoder.
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Membranes and padding for Western protein transfers, pre-cut
Supplier: G-Biosciences
Pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. Nitrocellulose (0,22 µm pore size) and PVDF (0,45 µm pore size) membranes are available.
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Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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Amersham™ Protran® Supported Nitrocellulose Western Blotting Membranes, Cytiva
Supplier: Cytiva
Amersham™ Protran® Supported is a robust protein blotting membrane, with 0,2 µm pore size, made of reinforced nitrocellulose for multiple reprobings.
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Blottingmembran, Amersham Hybond™-N+
Supplier: Cytiva
Amersham™ Hybond™-N+ är ett positivt laddat nylonmembran med en bindningskapacitet för nukleinsyror på upp till 600 µg/cm² och rekommenderas för användning med radioaktiva och icke radioaktiva detekteringssystem med kemiluminiscens eller kemifluorescens.
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Blotting membranes, Amersham™ Hybond™ SEQ 0,2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ SEQ 0.2 PVDF is a 0,2 µm pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane minimises ‘blow-through’ and increases protein binding over a wide range of molecular weights.
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Blotting membranes, Amersham™ Hybond™ LFP 0,2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ LFP 0.2 PVDF is a low fluorescent, hydrophobic membrane, with 0,2 µm pore size, for protein transfer. Amersham™ Hybond™ LFP is optimised for fluorescent detection in Western blotting applications and for use with the Amersham™ ECL Plex™ detection system.
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Amersham™ Protran® Premium Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® Premium nitrocellulose blotting membranes provide excellent sensitivity, resolution and low background in fluorescence Western blotting detection applications.
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Blotting membranes, Amersham™ Hybond™ 0.2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ 0.2 PVDF is a 0,2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimises 'blow-through' and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of
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Amersham™ Protran® Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
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Blottingmembran, Amersham Hybond™ ECL™
Supplier: Cytiva
Hybond™ ECL™ ger utmärkt känslighet och upplösning samt låg bakgrund för alla märknings- och detektionssystem (radioaktiva, icke radioaktiva och kromogena), i synnerhet tillsammans med ECL™, ECL™ Prime och ECL™ Plex Western-detektionssystemen och detektionsreagens. Specialförpackning med skydd på båda sidor om membranen och lufttät, återförslutbar aluminiumpåse med plats för anteckning av öppningsdatum.
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Blotting membrane, nitrocellulose
Supplier: Sartorius
These nitrocellulose blotting membranes are ideal for Western blotting, DNA blotting, as well as dot or slot blots. They are optimally adapted to all protein blotting systems, such as electro transfer, semi-dry or simple capillary blotting. A high protein binding capacity is combined with low background to give good blocking possibilities. High membrane stability ensures easy handling of the blotting membrane, even when wet.