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77 resultat för Proteinanalys

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Proteinanalys

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

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Firefly luciferase flash assay kit, Pierce™

Firefly luciferase flash assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Supplier: Merck Millipore (Novagen)

The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

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ReadiUse™ TCEP Removal Solution

ReadiUse™ TCEP Removal Solution

Supplier: AAT BIOQUEST

TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.

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GST protein interaction pull down kit, Pierce™

GST protein interaction pull down kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

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Amplite® Rapid Colorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.

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Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

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Phosphoprotein phosphate estimation assay Kit, Pierce™

Phosphoprotein phosphate estimation assay Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

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Horseradish Peroxidase, Biotinylated, Pierce™

Horseradish Peroxidase, Biotinylated, Pierce™

Supplier: Thermo Fisher Scientific

Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.

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Amplite® Fluorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

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Protein Assay, Protn-Latex™

Protein Assay, Protn-Latex™

Supplier: G-Biosciences

A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.

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Non-interfering Protein Assay™ kit, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.

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Quantitative colorimetric peptide assay, Pierce™

Quantitative colorimetric peptide assay, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

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Glutathione Fluorometric Assay Kit

Glutathione Fluorometric Assay Kit

Supplier: Abcam

Simple assay to detect total glutathione.

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Streptavidin poly-HRP conjugate, Pierce™

Streptavidin poly-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

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Biotin labelling kit

Biotin labelling kit

Supplier: Abnova

Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.

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2-D Quant kit

2-D Quant kit

Supplier: Cytiva

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

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Histone, from calf thymus, MP Biomedicals

Supplier: MP Biomedicals

Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

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