Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Pierce™ GlycoProtein Carbohydrate Estimation Kits
Supplier: Thermo Fisher Scientific
Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.
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Surface protein (allergen) test swab ALLTEC60, Clean-Trace™
Supplier: 3M Food Safety
3M™ Clean-Trace™ Surface Protein (Allergen) Test Swab ALLTEC60 is a rapid, simple, and reliable solution to monitor the presence of allergens on surfaces to effectively manage cleaning.
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Surface protein plus test swabs, PRO100, Clean-Trace™
Supplier: 3M Food Safety
3M™ Clean-Trace™ Surface Protein Plus Test Swab PRO100 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.
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Lumiwox™ Acridinium NHS Ester
Supplier: AAT BIOQUEST
The acridinium NHS ester can be used to label proteins and nucleic acids.
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ReadiUse™ TCA Deproteinization Sample Preparation Kit
Supplier: AAT BIOQUEST
The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates.
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Tetracysteine FLASH Reagent *2 mM DMSO Solution*
Supplier: AAT BIOQUEST
FLASH is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.
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Bradford protein assay kits (B-Pak)
Supplier: OZ BIOSCIENCES
Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.
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Octet® Anti-penta-HIS (HIS1K) biosensors
Supplier: Sartorius
For label-free quantitation and kinetic analysis of his-tagged proteins.
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Coomassie (Bradford) protein assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
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Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Tetracysteine REASH Reagent *2 mM DMSO Solution*
Supplier: AAT BIOQUEST
REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.
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Biotinylated-protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.
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Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible
Supplier: Thermo Fisher Scientific
This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).
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Pierce™ Protease Assay Kits, Colorimetric and Fluorometric
Supplier: Thermo Fisher Scientific
Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.
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Surface protein plus test swabs, MPR050, Clean-Trace™
Supplier: 3M Food Safety
3M™ Clean-Trace™ Surface protein plus test swab MPRO50 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.
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Pierce™ 660 nm Protein Assay Kit and Reagent
Supplier: Thermo Fisher Scientific
Pierce™ 660 nm Protein Assay is a ready to use, detergent- and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a protein standard.
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Allergen protein rapid test kits
Supplier: 3M Food Safety
The 3M™ Allergen Protein Rapid Kits are intended for screening for the presence of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
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Compat-Able™ BCA™ Protein assay kit
Supplier: Thermo Fisher Scientific
The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.
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Pierce™ Biotin Quantitation Kit
Supplier: Thermo Fisher Scientific
Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
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Firefly luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
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ApoSight™ Green Caspase 3/7 substrate
Supplier: AAT BIOQUEST
ApoSight™ Green Caspase 3/7 substrate is the first fluorogenic probe for the direct fluorescence imaing of caspase activities in live cells.
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Pierce™ Micro BCA™ Protein Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.
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Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™
Supplier: Thermo Fisher Scientific
Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
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Compat-Able™ Protein assay preparation reagent set
Supplier: Thermo Fisher Scientific
The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
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Coomassie Plus™ protein assay kit, Compat-Able™
Supplier: Thermo Fisher Scientific
The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit
Supplier: Merck Millipore (Novagen)
The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
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Firefly luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
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Pierce™ BCA™ Protein assay kits and reagents
Supplier: Thermo Fisher Scientific
Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
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Modified lowry protein assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.
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Quantitative fluorometric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.