Du sökte efter: System för proteinlänkning och märkning

Supplier: VWR Chemicals

Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.

Supplier: G-Biosciences

A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

Supplier: ENZO LIFE SCIENCES

Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.

Supplier: G-Biosciences

The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.

Supplier: Rockland Immunochemicals

Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

Supplier: Apollo Scientific

Liquid product colourless odourless pH-value 5,9 to 6,1, Stabiliser for very low concentrated AP conjugates. Suitable for proteins resistant to pH values <6,0.

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.

Supplier: Biotium

Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.

Supplier: Biotium

CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

Supplier: Biotium

CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

Supplier: ENZO LIFE SCIENCES

Cell viability dye

Supplier: ENZO LIFE SCIENCES

Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

Supplier: ENZO LIFE SCIENCES

The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.

Supplier: ENZO LIFE SCIENCES

Organelle-ID RGB® reagent IV is a mixture of cell-permeable red fluorescent endoplasmic reticulum dye, green fluorescent lysosomal dye, and blue fluorescent nucleic acid dye.

Supplier: G-Biosciences

HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

Supplier: Biotium

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

Supplier: Biotium

Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

Supplier: Biotium

A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Supplier: G-Biosciences

CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

Supplier: Biotium

Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Supplier: Biotium

Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

Supplier: ENZO LIFE SCIENCES

Orange 552 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.

Supplier: ENZO LIFE SCIENCES

TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.

Supplier: Biotium

Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

Supplier: Biotium

Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.

Supplier: ENZO LIFE SCIENCES

Fluorescent probe for Zn2+

Supplier: Apollo Scientific

Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.

Supplier: G-Biosciences

A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.