Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.

Supplier: G-Biosciences

HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.

Supplier: G-Biosciences

FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.

Supplier: AAT BIOQUEST

XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher.

Supplier: Thermo Fisher Scientific

ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Supplier: G-Biosciences

HOOK™-PEG₂-Iodoacetyl-biotin, 50 mg

Supplier: Thermo Fisher Scientific

The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised reductant column contains beaded resin to which an thiol-based reducing agent has been immobilised to enable solid-phase reduction of peptide and protein disulphide bonds.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

Supplier: AAT BIOQUEST

The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

Supplier: AAT BIOQUEST

Cy3 is one of the most popular fluorescent labelling dyes for preparing orange-red fluorescent bioconjugates.

Supplier: AAT BIOQUEST

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC and iFluor® tandem dyes.

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 647 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

Supplier: AAT BIOQUEST

iFluor® 750 labelled WGA exhibits a distinct far-red fluorescence characteristic, emitting maximally at 779 nm.

Supplier: AAT BIOQUEST

Cy3-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.

Supplier: AAT BIOQUEST

XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.

Supplier: Thermo Fisher Scientific

The iodoTMTzero™ label reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation.

Supplier: Thermo Fisher Scientific

The iodoTMTsixplex™ Isobaric Mass Tag Labeling Kit contains iodoTMT™ reagents and associated materials for the irreversible labeling of sulfhydryl groups.

Supplier: Thermo Fisher Scientific

Pierce™ Magnetic RNA-Protein Pull-Down Kit is a streamlined, robust method to enrich protein-RNA interactions using end-labelled RNA as the bait.

Supplier: Thermo Fisher Scientific

Pierce™ RNA 3' End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Supplier: Thermo Fisher Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used cross linkers for these applications.

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS.