Du sökte efter: System för proteinlänkning och märkning
Protein linking eller labelling kit underlättar koppling av fluorescerande markörer eller biotiner mot antikroppar och större proteiner. Dessa kit är idealiska för användning inom ett antal applikationer, såsom flödescytometri, fluorescerande mikroskopi, immunhistokemi, primär detektion, ELISA, immuncytokemi och FISH. Labelling och linking kits finns med olika färgämnen, biotin, fluorescerande reagenser och alla komponenter som behövs för att utföra multipla konjugeringar och reningar. Proteinlabelling följer ett enkelt protokoll för reaktion, separation och användning.
iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.
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HIS Lite™ iFluor® 568 Tris NTA Chelator
Supplier: AAT BIOQUEST
iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.
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HIS Lite™ iFluor® 647 Tris NTA-Ni Complex
Supplier: AAT BIOQUEST
iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.
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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labelling Kit
Supplier: AAT BIOQUEST
Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.
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DY677 CEP (DyLight 677 Phosphoramidite)
Supplier: AAT BIOQUEST
DY677 CEP (DyLight 677 phosphoramidite) is an excellent replacement to the commonly used Cy5.5 phosphoramidite.
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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
APC-iFluor® 700 Tandem is an excellent replacement for APC-Alexa Fluor® 700 Tandem since they have almost identical spectra.
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Buccutite™ Rapid APC-Cy7 Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
APC-Cy7 is a popular colour used in flow cytometry.
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Buccutite™ MTA, NHS ester (2 µmoles)
Supplier: AAT BIOQUEST
Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.
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ReadiLink™ Rapid XFD594 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).
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ReadiLink™ Rapid XFD488 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).
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ReadiLink™ Rapid iFluor® 350 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.
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ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.
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ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)
Supplier: AAT BIOQUEST
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
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MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria
Supplier: AAT BIOQUEST
Oxidation of MitoROS™ 580 by superoxide generates red fluorescence.
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ReadiLink™ Rapid iFluor® Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.
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ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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Wheat Germ Agglutinin, XFD647 Labelled *XFD647 Same Structure to Alexa Fluor® 647*
Supplier: AAT BIOQUEST
XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher.
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Carboxyl reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.
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Carbohydrate reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.
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Dye labeling kit (FITC), HOOK™
Supplier: G-Biosciences
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.
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ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit
Supplier: AAT BIOQUEST
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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DY547 CEP (DyLight 547 Phosphoramidite)
Supplier: AAT BIOQUEST
DY547 CEP (DyLight 547 phosphoramidite) is an excellent replacement to the commonly used Cy3 phosphoramidite.
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Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kits (2 Labelings)
Supplier: AAT BIOQUEST
Protein-protein conjugations are commonly performed with a bifunctional linker (such as SMCC) having different reactivity at each end for linking two different proteins.
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ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit
Supplier: AAT BIOQUEST
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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PSA™ iFluor® 594 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Buccutite™ FOL scavenger (2 µmoles)
Supplier: AAT BIOQUEST
Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.
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HEX-dT Phosphoramidite
Supplier: AAT BIOQUEST
HEX-dT phosphoramidite provides a valuable tool to add the HEX tag to a desired location of an oligo sequence.
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Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction* (2 Labelings)
Supplier: AAT BIOQUEST
Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC.