System för proteinlänkning och märkning
Protein linking eller labelling kit underlättar koppling av fluorescerande markörer eller biotiner mot antikroppar och större proteiner. Dessa kit är idealiska för användning inom ett antal applikationer, såsom flödescytometri, fluorescerande mikroskopi, immunhistokemi, primär detektion, ELISA, immuncytokemi och FISH. Labelling och linking kits finns med olika färgämnen, biotin, fluorescerande reagenser och alla komponenter som behövs för att utföra multipla konjugeringar och reningar. Proteinlabelling följer ett enkelt protokoll för reaktion, separation och användning.
IgG Biotinylation kits, HOOK™
Supplier: G-Biosciences
HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.
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PerCP/Cy5.5® Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using PerCP/Cy5.5® Conjugation Kit - Lightning-Link® ab102911. 100% antibody recovery.
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Heparin Beads 6FF, Abcam
Supplier: Abcam
Supplemental Information * Ligand: Heparin * Basic Beads: 6% Cross-linked agarose beads * Particle size: 45μm – 165μm * Storage Buffer: 80% PBS, 20% ethanol * pH Stability Range: 4-12 * Maximum pressure: 0.3 MPa * Binding Capacity: >20mg DNA polymerase/ml .
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Biotin picolyl azide
Supplier: Biotium
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
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PerCP Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
PerCP conjugation / labeling in < 4 hrs with 30 secs hands-on time using PerCP Conjugation Kit - Lightning-Link® ab102907. 100% antibody recovery.
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DyLight® 594 Conjugation Kit (Fast) - Lightning-Link®, Abcam
Supplier: Abcam
DyLight® 594 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 594 Conjugation Kit (Fast) - Lightning-Link® ab201801. 100% antibody recovery.
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CDI amine reactive resin
Supplier: G-Biosciences
CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
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Antibody Purification Kit (Protein A), Abcam
Supplier: Abcam
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.
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153Eu Metal Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using 153Eu Metal Conjugation Kit - Lightning-Link® ab269836. 100% antibody recovery.
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CF® Dye dCTP conjugates
Supplier: Biotium
CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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HRP Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Abcam's HRP Conjugation Kit (ab102890): Fast, Efficient, and Reliable - Rapid Conjugation: Achieve HRP conjugation in under 4 hours with just 30 seconds of hands-on time using the HRP Conjugation Kit ab102890. - High Efficiency: Ensures 100% antibody recovery, preserving the integrity and value of your antibodies. - Effortless Protocol: Add modifier to your antibody, incubate for 3 hours, then add quencher for 30 minutes, with no need for further purification. - Scalable: Compatible with most standard antibody formulations, effective for 10µg to 100mg of antibody. - Versatile Conjugation: Utilize Lightning-Link® technology to conjugate with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles. - Confidence: cited in over 60 publications
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APC/Cy7® Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using APC/Cy7® Conjugation Kit - Lightning-Link® ab102859. 100% antibody recovery.
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AP-conjugate stabiliser, SeramunStab® STAP
Supplier: Apollo Scientific
Liquid product colourless odourless pH-value 5,9 to 6,1, Stabiliser for very low concentrated AP conjugates. Suitable for proteins resistant to pH values <6,0.
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Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit, Abcam
Supplier: Abcam
AZdye 488 conjugation / labeling in < 4 hrs with 30 secs hands-on time using Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit ab272187. 100% antibody recovery.
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5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30, Abcam
Supplier: Abcam
Oligonucleotide conjugation / labeling in < 4 hrs with 30 secs hands-on time using 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 ab270709. 100% antibody recovery.
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CF™ azide reactive dyes
Supplier: Biotium
CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
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