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System för proteinlänkning och märkning

System för proteinlänkning och märkning

Protein linking eller labelling kit underlättar koppling av fluorescerande markörer eller biotiner mot antikroppar och större proteiner. Dessa kit är idealiska för användning inom ett antal applikationer, såsom flödescytometri, fluorescerande mikroskopi, immunhistokemi, primär detektion, ELISA, immuncytokemi och FISH. Labelling och linking kits finns med olika färgämnen, biotin, fluorescerande reagenser och alla komponenter som behövs för att utföra multipla konjugeringar och reningar. Proteinlabelling följer ett enkelt protokoll för reaktion, separation och användning.

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PE/Cy7® Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

The Abcam PE/Cy7® Conjugation Kit ( ab102903) offers several standout features: - Rapid Conjugation: Achieve PE/Cy7® labeling in under 4 hours with just 30 seconds of hands-on time. - High Efficiency: Ensures 100% antibody recovery, preserving the integrity and value of your antibodies. - Versatility: Suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence- cited in over 15 publications.

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PE/Cy5® Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

The Abcam PE/Cy5® Conjugation Kit ( ab102893) offers several standout features: - Rapid Conjugation: Achieve PE/Cy5® labeling in under 4 hours with just 30 seconds of hands-on time. - High Efficiency: Ensures 100% antibody recovery, preserving the integrity and value of your antibodies. - Versatility: Suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification.

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Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Alexa Fluor® 647 antibody labeling/conjugation kit, Lightning-Link® requires < 20 minutes with 30 seconds of hands-on time. 100% antibody recovery. - Cited in over 40 publications - 15-minute incubation time - Alexa Fluor® 647 labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable; use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

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AMCA Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

AMCA conjugation / labeling in < 20 mins with 30 secs hands-on time using AMCA Conjugation Kit (Fast) - Lightning-Link® ab195224. 100% antibody recovery.

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Rhodamine Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Rhodamine conjugation / labeling in < 20 mins with 30 secs hands-on time using Rhodamine Conjugation Kit (Fast) - Lightning-Link® ab188286. 100% antibody recovery.

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Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®, Abcam

Supplier: Abcam

Biotin conjugation / labeling in < 20 mins with 30 secs hands-on time using Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® ab201796. 100% antibody recovery - Cited in over 15 publications - Biotin labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable;use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

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DyLight® 594 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

DyLight® 594 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 594 Conjugation Kit (Fast) - Lightning-Link® ab201801. 100% antibody recovery.

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TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green), Abcam

Supplier: Abcam

Abcam's TripleStain IHC Kit (ab183290) is designed to be used with user-supplied one goat, one mouse and one rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples.

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Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®, Abcam

Supplier: Abcam

Alkaline Phosphatase conjugation / labeling in < 4 hrs with 30 secs hands-on time using Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® ab269901. 100% antibody recovery.

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Lipophilic carbocyanine dyes and CellBrite cytoplasmic membrane labelling kits

Supplier: Biotium

Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

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490/515 nm Extracellular Vesicle Membrane Staining Kit, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 490/515 nm Extracellular Vesicle Membrane Staining Kit ab314686. 100% antibody recovery.

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Gold Conjugation Kit (20 nm, 20 OD), Abcam

Supplier: Abcam

20nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold Conjugation Kit (20 nm, 20 OD) ab188215. 100% antibody recovery.

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IgG Biotinylation kits, HOOK™

IgG Biotinylation kits, HOOK™

Supplier: G-Biosciences

HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

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CDI amine reactive resin

CDI amine reactive resin

Supplier: G-Biosciences

CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

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Antibody Purification Kit (Protein A), Abcam

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.

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APC Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

APC conjugation / labeling in < 4 hrs with 30 secs hands-on time using APC Conjugation Kit ab201807. 100% antibody recovery. - Cited in over 50 publications - APC labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable;use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

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