System för proteinlänkning och märkning
Protein linking eller labelling kit underlättar koppling av fluorescerande markörer eller biotiner mot antikroppar och större proteiner. Dessa kit är idealiska för användning inom ett antal applikationer, såsom flödescytometri, fluorescerande mikroskopi, immunhistokemi, primär detektion, ELISA, immuncytokemi och FISH. Labelling och linking kits finns med olika färgämnen, biotin, fluorescerande reagenser och alla komponenter som behövs för att utföra multipla konjugeringar och reningar. Proteinlabelling följer ett enkelt protokoll för reaktion, separation och användning.
Antibody labelling kits, Mix-n-Stain™ DNP
Supplier: Biotium
Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.
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High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.
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Ghost Dye™ Red 710
Supplier: Tonbo Biosciences
Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.
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Antibody labelling kits, Mix-n-Stain™ Digoxigenin
Supplier: Biotium
Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.
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Streptavidin, maleimide-activated
Supplier: Thermo Fisher Scientific
Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.
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Biotin kits, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
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Peptide coupling kits, HOOK™
Supplier: G-Biosciences
The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.
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Biotin NTA
Supplier: Biotium
Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.
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FluorSave™ reagent, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.
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Immunostainer enhancer, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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VeriFluor™ Far-Red Passive Reference Dye
Supplier: Biotium
A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.
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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)
Supplier: Cytiva
Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.
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Reactive Dye 5-packs, Amersham CyDye™
Supplier: Cytiva
Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.
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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva
Supplier: Cytiva
Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.
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Fluorescerande nukleotider, Amersham CyDye™
Supplier: Cytiva
Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.
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Fluorescent dyes, M series, streptavidin conjugate
Supplier: Abnova
Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
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