Proteinextraktions

Supplier: G-Biosciences

DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Supplier: Abcam

Lipid Extraction Kit (Chloroform-Free) provides a safer, chloroform-free method for efficient lipid isolation from plasma, serum, tissue, and cells. The recovered organic phase can be dried and resuspended for downstream lipid analysis. - Cited in over 15 publications - Supports multiple sample types including - plasma, serum, tissue, suspension cells, and adherent cells. - Chloroform-free - eliminates the use of toxic chloroform in lipid extraction - Efficient lipid isolation – high-yield lipid extraction with minimal contamination - Sufficient for 50 lipid preparations (25 µL sample size)

Supplier: Abcam

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.

Supplier: G-Biosciences

FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

Supplier: Abcam

Mitochondria isolation kit for tissue is designed for the efficient isolation of mitochondria from hard or soft tissues, providing high-quality mitochondria for downstream analysis. The kit contains enough reagents for up to 10 isolations. - Cited in over 65 publications - Isolates mitochondria from both hard and soft tissues, such as brain, liver, and heart - Suitable for western blotting, immunocapture, or activity assays - Simple Protocol – no need for ultracentrifugation, making the process more efficient and user-friendly

Supplier: Abcam

Mitochondria isolation kit for cultured cells is designed for efficient isolation of mitochondria from cultured mammalian cells. The kit provides enough reagents for up to 20 isolations and is suitable for analyzing mitochondria by Western blotting, immunocapture, or activity assays. - Cited in over 75 publications - Isolates mitochondria from cultured mammalian cells with ease - Useful in Western blotting, immunocapture, or mitochondrial activity assays - Sufficient reagents for up to 20 benchtop mitochondria isolations

Supplier: Abcam

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

Supplier: Thermo Fisher Scientific

The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Supplier: Abcam

Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Supplier: Abcam

Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins. - Cited in over 200 publications - High yield and purity – consistent yield with high purity (over 90%) - Suitable for western blotting, 2-D gels, enzyme analyses, and more

Supplier: G-Biosciences

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

Supplier: Thermo Fisher Scientific

The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

Supplier: Thermo Fisher Scientific

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

Supplier: Abcam

60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format. - Cited in over 50 publications