Buffertar

Buffertar skyddar prover mot pH-förändringar när syror eller alkalier tillsätts. Pulver- och flytande former spårar effektivt proteiner under elektroforesisolering och separation. Saltbuffertarna kan också användas för att kalibrera och standardisera pH-mätare för noggranna avläsningar. Blockerande ämnen hindrar antikroppar från att fästa vid membranfria platser som skulle kunna producera en störande bakgrund. Buffertarna finns i olika koncentrationer för att passa alla behov och kan komma i flytande eller dehydrerade format.

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RoboSep™-C PBS/EDTA Buffer

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Ambion

Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)

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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Molekula

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Molekula

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Ammonium Chloride Solution

Supplier: Stemcell Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Intracellular Permeabilisation Buffer (10X)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++

Supplier: Stemcell Technologies

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Buffer, TE solution (TRIS-EDTA buffer) pH 7,5

Supplier: Molekula

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,5

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

Supplier: Molekula

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

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Ambion® Buffer, TE solution (TRIS-EDTA buffer) pH 8,0, RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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Ambion® buffer, sodium acetate buffer solution pH 5,5 (3 mol/L), RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, Sodium acetate buffer solution pH 5,5 (3 mol/l)

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Ambion® Buffer, TE solution (TRIS-EDTA buffer), RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)

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EasySep™ Red Blood Cell Lysis Buffer

Supplier: Stemcell Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.