Buffertar

Buffertar skyddar prover mot pH-förändringar när syror eller alkalier tillsätts. Pulver- och flytande former spårar effektivt proteiner under elektroforesisolering och separation. Saltbuffertarna kan också användas för att kalibrera och standardisera pH-mätare för noggranna avläsningar. Blockerande ämnen hindrar antikroppar från att fästa vid membranfria platser som skulle kunna producera en störande bakgrund. Buffertarna finns i olika koncentrationer för att passa alla behov och kan komma i flytande eller dehydrerade format.

Laemmli SDS sample buffer, non-reducing (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 6X

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Kinase buffer I (5X)

Supplier: Thermo Fisher Scientific

Kinase buffer I (5X)

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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8,3

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-Tricine-SDS running buffer (10X), cathode buffer

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NP-40 lysis buffer

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, Thermo Fisher Scientific

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PIPES-buffered saline (5X), pH 6,5

Supplier: Thermo Fisher Scientific

PIPES-buffered saline, 5X, pH-värde: 6,5, Colourless, Liquid

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Phosphate, 0,2 M buffer solution, pH 8,0

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH-värde: 8,0, Liquid

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Phosphate, 0,2 M buffer solution, pH 4,4

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH-värde: 4,4, Liquid

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IEF Cathode buffer (10X) with lysine and arginine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine and arginine

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Fosfat, pH-värde: 7,00, ±0.02, Referenstemperatur: 20 °C, Fluka™, 6×1 L

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Triethylammonium acetate buffer, BioUltra, volatile buffer, Fluka®

Supplier: Honeywell Chemicals

Triethylammonium acetate buffer, 1,0 M in H₂O, pH-värde: 7,0

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Laemmli SDS sample buffer, non-reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X

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GST Buffer Kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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Buffer Capsules

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.