Buffertar
Buffertar skyddar prover mot pH-förändringar när syror eller alkalier tillsätts. Pulver- och flytande former spårar effektivt proteiner under elektroforesisolering och separation. Saltbuffertarna kan också användas för att kalibrera och standardisera pH-mätare för noggranna avläsningar. Blockerande ämnen hindrar antikroppar från att fästa vid membranfria platser som skulle kunna producera en störande bakgrund. Buffertarna finns i olika koncentrationer för att passa alla behov och kan komma i flytande eller dehydrerade format.
ACK Lysing Buffer (1x), TheraPEAK™
Supplier: LONZA
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.
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Buffers, citrate, 0,5 M solutions
Supplier: Thermo Fisher Scientific
Citrate, buffer solution, 0,5 M, pH-värde: 5,5, Colourless, Odourless, Liquid
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Buffers, tricine, 0,5 M solutions
Supplier: Thermo Fisher Scientific
Tricine, 0,5M buffer solution, pH-värde: 6,5, Colourless, Liquid
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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific
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Succinate, 0,2 M buffer solution, pH 5,0
Supplier: Thermo Fisher Scientific
Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific
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Laemmli SDS sample buffer with pyronin Y, reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, with PyroninY, 4X
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Buffer, HEPES solution pH 7,3 (1 mol/L), Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
HEPES buffer solution pH 7.3 (1 mol/l)
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TES, 0,2 M buffer soln., pH 7,5
Supplier: Thermo Fisher Scientific
TES buffer solution, 0,2 M, pH-värde: 7,5, Liquid
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Glycine, 0.2M buffer soln., pH 2.5
Supplier: Thermo Fisher Scientific
Glycine buffer solution, 0,2 M, pH-värde: 2,5, Odourless, Liquid
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Thermo Fisher Scientific
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L) AccuGENE®
Supplier: LONZA
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l) AccuGENE®
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Buffer solutions, colour coded, Fluka™
Supplier: Honeywell Chemicals
These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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IEF Anode buffer (50X)
Supplier: Thermo Fisher Scientific
Running Buffer, IEF Anode buffer (50X)
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals