Buffertar

Buffertar skyddar prover mot pH-förändringar när syror eller alkalier tillsätts. Pulver- och flytande former spårar effektivt proteiner under elektroforesisolering och separation. Saltbuffertarna kan också användas för att kalibrera och standardisera pH-mätare för noggranna avläsningar. Blockerande ämnen hindrar antikroppar från att fästa vid membranfria platser som skulle kunna producera en störande bakgrund. Buffertarna finns i olika koncentrationer för att passa alla behov och kan komma i flytande eller dehydrerade format.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.

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Buffertlösning, Certipur®, färgkodad, Supelco®

Supplier: Merck

Material för exakt kalibrering och kontroll av pH-mätare.

Sustainable Options Available

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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations, Supelco®

Supplier: Merck

TISAB III solution for fluoride determinations

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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Supplier: VWR Chemicals

Tris (hydroxymethyl) aminomethane.

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VWR®, bufferttabletter för hematologi

Supplier: VWR Chemicals

These tablets provide a rapid and convenient method of preparing a buffer solution.

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J.T.Baker® DILUT-IT™, Buffer Solutions, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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MOPS-buffert

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Sustainable Options Available

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Natriumfosfat, lösningsmediakoncentrat, DILUT-IT™

Supplier: Avantor

DILUT-IT™ lösningsmediakoncentrat, natriumfosfat, 961,5 ml, J.T.Baker® DILUT-IT®, pH-värde: 6,8

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VWR®, EDTA buffer solution pH 8,0 (0,5 mol/L) for biotechnology

Supplier: VWR Chemicals

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l) for biotechnology

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VWR® UltraPure, Phosphate buffered saline (PBS), powdered

Supplier: VWR Chemicals

PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.