Du sökte efter: Cellodling-supplement och -tillsatser
Supplement, tillskott eller tillsatser för cellkuturer kompenserar egenskaper som basmediet saknar för att ge maximal celltillväxt. Att tillföra supplement under utveckling ger stabilitet och maximal aktivitetskontroll till cellodlingar. De tillsatta stabiliserande vätskorna stödjer cellproduktivitet, isolering och identifierig av mikroorganismer. Det finns ett brett sortiment av celllodlingstillskott och tillsatser i olika koncentrationer som ger möjlighet att hitta rätt ämne med kompatibilitet även för känsliga medier och cellodlingar.
Fosfatas hämmare, Halt
Supplier: Thermo Fisher Scientific
Fosforylering och defosforylering är en molekylär på/av-knapp som reglerar ett antal viktiga biologiska processer i cellen som signaltransduktion, celldelning och apoptos. Halts fosfatas hämmarblandning bevarar fosforyleringen av proteiner i cellysat och vävnadsextrakt.
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Matrix, CELLvo™
Supplier: STEMBIOSYS
CELLvo™ Matrix is a ready to use cell culture substrate which provides a native microenvironment that replicates the 3-dimensional 'home' in which stem cells naturally reside and proliferate.
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Serum replacement, Corning® Nu-Serum™ IV
Supplier: Corning
Corning® Nu-Serum™ IV odlingsmediatillsats ger ett låg-proteinalternativ till nyfödd kalv, fetalbovin och andra sera som rutinanvänds för cellodling.
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Yeast extract, BioChemica
Supplier: PanReac AppliChem
Yeast extract BioChemica
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Serum-free/low-protein medium, 1X with L-glutamine and 1 g/L BSA, Corning®
Supplier: Corning
Corning® cellgro® COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy's 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0 g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspension and adherent cell cultures.
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Corning® Interleukin-3 (IL-3) Culture Supplement, Corning
Supplier: Corning
Interleukin-3 (IL-3) is a glycoprotein that regulates differentiation and enhances proliferation of a variety of hematopoietic precursor cells, mast cells, basophils, and NK cells.
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Corning® Endothelial Cell Growth Supplement, Corning
Supplier: Corning
Endothelial Cell Growth Supplement (ECGS) är ett brett använt supplement vid odling av en rad olika celler, särskilt endotelceller.
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Protease inhibitor tablets, EDTA-free, Pierce™
Supplier: Thermo Fisher Scientific
These inhibitor tablets contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.
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Corning® T-Cell Culture Supplement with ConA (IL-2 Culture Supplement), Corning
Supplier: Corning
T-STIM with ConA (IL-2 culture supplement), rat, is used to promote proliferation and activation of T-cells and support high-titer HIV production by leukocytes.
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Corning® ITS Universal Culture Supplement Premix, Corning
Supplier: Corning
ITS contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media.
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Corning® MITO+ Serum Extender, Corning
Supplier: Corning
Corning® MITO+ Serum Extender är en koncentrerad, helt definierad sammansättning av hormoner, tillväxtfaktorer (EGF och FGF) och andra metaboliter (insulin- och steroidhormoner).
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Corning® Bovine Pituitary Extract (BPE), Corning
Supplier: Corning
Bovine Pituitary Extract (BPE) är en brett använd tillsats för att odla en mängd epiteliala och endoteliala celler.
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MEM vitamin solution, 100X, Corning®
Supplier: Corning
The addition of MEM vitamins to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.
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Cell Recovery Solution, Corning®
Supplier: Corning
Corning® Cell Recovery Solution möjliggör återvinning av celler odlade på Corning® Matrigel® Basement Membrane Matrix för påföljande biokemisk analys. Corning® Cell Recovery Solution depolymeriserar Corning® Matrigel® Matrix-geler utan enzymatisk behandling och långdragna inkuberingsperioder i höga temperaturer. Cellerna frigörs utan skada, och undviker därmed biokemiska förändringar under inkubering och nedbrytning av extracellulära delar av cellytans receptorer och vidhäftningsmolekyler.
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3D Clear tissue clearing reagents and buffers, Corning®
Supplier: Corning
In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.
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Matris med hög koncentration, Corning® Matrigel®
Supplier: Corning
Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.
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Phosphatase inhibitor cocktail II
Supplier: Thermo Fisher Scientific
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts.
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Supplement, Corning® glutagro™
Supplier: Corning
Cells grown in traditional media supplemented with L-glutamine are susceptible to toxicity.
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MEM Nonessential amino acids, Corning®
Supplier: Corning
The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.
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Invasionskammare, Corning® BioCoat™ Matrigel®
Supplier: Corning
Ett in vitro-system för bedömning av den invasiva potentialen hos både maligna och normala celler.
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Protease Inhibitor Cocktail, Animal-Free, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
Calbiochem® protease inhibitor cocktail set III, animal-free, is a cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine and serine proteases and aminopeptidases.
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HyClone Cell Boost™ Process Supplement Kit
Supplier: HyClone products (Cytiva)
The HyClone Cell Boost™ process supplement kit contains 6×100 g samples of supplements designed to increase cell productivity in a variety of cell lines. Each supplement is designed to provide nutrients to fed-batch culture applications.
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Corning® Matrigel® hESC-Qualified Matrix, Corning
Supplier: Corning
Pre-screned for compatibility with mTeSR™1 medium by StemCell Technologies providing the reproducibility and consistency essential for hESC and iPSC feeder-free stem cell culture.
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Corning® Matrigel® Basement Membrane Matrix, Corning
Supplier: Corning
Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.
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Corning® Matrigel®-matrisplattor
Supplier: Corning
96-well plates, coated, PS non-sterile, flat bottom, Corning® Matrigel®
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SC substrate, Corning® Synthemax® II
Supplier: Corning
Synthemax® II-SC Substrate is an easy to use, self-coating material for creating a unique synthetic surface that mimics the natural cell environment. This surface supports the adhesion and expansion of stem cells in their undifferentiated state and directs differentiation into specialised cell types. In addition, it offers a synthetic, xeno-free alternative to biological coatings and/or feeder cell layers used in hES and iPS cell culture, whenever needed and appropriate.
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HAT (Hypoxanthine, Aminopterin, Thymidine), Corning®
Supplier: Corning
HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells
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Phosphatase inhibitor cocktail I
Supplier: Thermo Fisher Scientific
Phosphatase Inhibitor Cocktail I is used to inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases.
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Insulin-Transferrin-Selenium (ITS) 100x Growth Supplement, Corning®
Supplier: Corning
Animal free formulation
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MEM amino acids 50X solution without L-glutamine, Corning®
Supplier: Corning
The addition of MEM nonessential amino acids to medium can improve growth and the viability of propagating cells in culture while reducing the biosynthetic burden on these cells.