Du sökte efter: Cellodling-supplement och -tillsatser
Supplement, tillskott eller tillsatser för cellkuturer kompenserar egenskaper som basmediet saknar för att ge maximal celltillväxt. Att tillföra supplement under utveckling ger stabilitet och maximal aktivitetskontroll till cellodlingar. De tillsatta stabiliserande vätskorna stödjer cellproduktivitet, isolering och identifierig av mikroorganismer. Det finns ett brett sortiment av celllodlingstillskott och tillsatser i olika koncentrationer som ger möjlighet att hitta rätt ämne med kompatibilitet även för känsliga medier och cellodlingar.
HyClone Cell Boost™ 1 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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Protease inhibitor cocktail, Yeast/Fungal ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases.
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VWR® Egg Yolk Emulsion, Sterile
Supplier: VWR Chemicals
Egg yolk emulsion, sterile, VWR®
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Protease hämmare, Halt
Supplier: Thermo Fisher Scientific
Halts protease hämmare erbjuder ett brett skyddsspektrum i en enda bruksfärdig vätska. Halt skyddar mot proteinnedbrytning initierad av serin, cystein och asparaginsyra-proteaser och aminopeptidaser. Metalloproteaser hämmas genom tillvald användning av 0,5 M EDTA. Halt 100X-stamlösning gör det enkelt att dispensera önskad mängd (10 µl per 1 ml) proteashämmare i buffert eller lyseringsreagens före proteinextraktion från odlade celler, djur- och växtvävnad, jäst eller bakterier. Halts proteas hämmarblandning är kompatibel med reagens för proteinextraktion från Thermo Scientific Pierce. Den EDTA-fria sammansättningen från Halt är idealisk för beredning av prover som ska analyseras med 2-D-gelelektrofores, immobiliserad metallkelat-affinitetskromatografi (IMAC) och andra nedströms tillämpningar där kelaterande medel ingår.
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Protease inhibitor cocktail, TCM-ProteaseArrest™
Supplier: G-Biosciences
TCM-ProteaseArrest™ is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic (acid) proteases and aminopeptidases.
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Kinase inhibitor library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well™ Kinase Inhibitor Library contains 80 known kinase inhibitors of well-defined activity. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 or 500 ml per well. The library is an ideal tool for chemical genomics, assay development and other pharmacological applications. Includes inhibitors of these important kinases: Insulin/IGF Receptors, PI 3-Kinase, CaM Kinase II, JAK, PKA, CDK, JNK, PKC, CKI II, MAPK, RAF, EGFR, MEK, SAPK, GSK, MLCK, Src-family, IKK, PDGFR, VEGFR and many more.
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Cholinergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The cholinergic ligands plate contains 72 compounds ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The cholinergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.
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Hematopoietic library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® hematopoietic toxicity library is a focused collection of 115 compounds with defined and diverse hematopoietic toxicity, including myelosuppression, neutropenia, leukopenia, anaemia, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.
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Protease inhibitor library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well™ Protease Inhibitor Library includes 53 known protease inhibitors on a single 96-well plate. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 or 500 microliters per well. The library includes inhibitors to a broad range of important proteases and is a convenient and cost-effective way to purchase a panel of protease inhibitors for chemical genomics, assay development, and other applications. It includes inhibitors of: Calpains, TPPII, Neutrophil Elastase, Cathepsins, DPPIV, Caspases, Granzyme B, gamma-Secretase, Furin, MMPs, ACE, Kallikrein, Proteasome, Aminopeptidase B, and many more.
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Opioid ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® opioid and sigma ligands plate contains 74 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The opioid and sigma ligands plate is 1 plate of the larger Neurotransmitter Library which contains over 650 ligands.
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Ion channel ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® ion channel ligand library contains 71 ion channel blockers and openers for use in characterising and identifying ion channels in individual cells or tissue.
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GABAergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The GABAergic igands library contains 56 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The GABAergics ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.
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Hydroxytyrosol Acetate
Supplier: ENZO LIFE SCIENCES
Hydroxytyrosol acetate is an antioxidant comparable to resveratrol. It is converted to hydroxytyrosol through hydrolysis by esterases.
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Metabotropic glutamatergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® metabotropic glutamatergic ligands plate contains 49 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The metabotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.
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Trypsin
Supplier: BIOWEST
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.
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L-alanyl L-glutamine, SG-200, HyClone™
Supplier: HyClone products (Cytiva)
SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.
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HyClone Cell Boost™ 6 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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Egg yolk tellurite emulsion
Supplier: THERMO FISHER DIAGNOSTICS
Egg yolk tellurite emulsion
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Protease inhibitor cocktail, Bacterial ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.
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Supplement Kit for Smooth Muscle Cell Growth Medium
Supplier: PROVITRO
The supplement kit designed for cultivating human smooth muscle cells (HSMC) provides an optimised formulation for promoting growth and maintaining the health of these cells. Upon adding the components of the supplement kit to a 500 ml basal medium, a specialised environment is created to support the culture of HSMCs. The formulation is specifically tailored for the initial seeding of 4000 cells per square centimeter, aiming to achieve confluence at approximately 90%. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. All components are subjected to rigorous biological assays. Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morphology, adherence rate, colony-forming efficiency, and population doubling time.
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Supplement Kit for MVEC Growth Medium, FCS
Supplier: PROVITRO
The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
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Endothelial Cell Proliferation Medium Supplement-Mix, FCS
Supplier: PROVITRO
The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.
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Phosphatase inhibitor library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well™ Phosphatase Inhibitor Library contains 33 known phosphatase inhibitors of well-defined activity. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 µl or 500 µl per well. The library is ideal for chemical genomics, assay development, and as a reference set for secondary screening. Includes inhibitors of these important phosphatases: Calcineurin(PP2B), JSP-1, PRL-1, CD45, PP1, PRL-3, CDC25, PP2A, PTEN, and many more.
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Wnt pathway library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® Wnt pathway library v 2.0 is a focused collection of 71 compounds with defined and diverse Wnt pathway activity, including activators and inhibitors of Wnts, Dishevelled, GSK-3β and other Wnt pathway kinases, TCF/β-catenin, DKK, LRP, Axin, Porcupine, and more. Compounds are dissolved in DMSO at 10 mM or 1 mM and aliquoted into deep-well plates at 100 or 500 µl per well. A variety of structurally and mechanistically different compound classes are included. A useful tool for studying the roles of pro- and anti-Wnt pathway molecules in cells as well as for use in in vitro applications.
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Adrenergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The adrenergic ligand library contains 84 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The adrenergic ligand library is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.
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Neurotransmitter library (10 plate set), Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® neurotransmitter library contains 681 CNS receptor ligands in a 96-well format. Ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, validating new assays, and for routine pharmacological applications.
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XF Matrix, CELLvo™
Supplier: STEMBIOSYS
CELLvo™ XF Matrix is a ready to use cell culture substrate which provides a xeno-free native microenvironment that replicates the 3-dimensional 'home' in which stem cells naturally reside and proliferate.
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Matrix Plus, CELLvo™
Supplier: STEMBIOSYS
CELLvo™ Matrix Plus is a substrate that aims to recapitulate the biochemical, structural and mechanical cues of the native niche by leaving the extracellular matrix intact. Cell-derived matrices create a biologically relevant culture environment for a variety of cell types including pluripotent cells by providing all the cues cells expect to receive in vivo in culture dishes.
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Cell preservation media, HypoThermosol®
Supplier: Charles River Laboratories Cell Solutions
HypoThermosol® biopreservation media is a novel, engineered, optimised hypothermic storage and shipping media product. Serum-free, protein-free HypoThermosol® is designed to provide maximum storage and shipping stability for biologics at 2 to 8 °C.