You searched for: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
Hybridoma growth and expression media HYGM6
Supplier: BIOCONCEPT
Hybridoma growth media (HYGM) 6 is a serum-free and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.
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Cancer Stem Cell Medium (BIT-100)
Supplier: PROVITRO
Cancer Stem Cell Medium is a sterile liquid culture medium designed for the cultivation of human cancer stem cells. Delivered in the form of a basal medium, it becomes a complete and effective environment for the growth of human cancer stem cells upon the addition of supplement mix components.
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Cell culture media, RPMI 1640
Supplier: BIOWEST
RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.
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Cell culture media, DMEM-Ham's F12
Supplier: BIOWEST
Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.
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Hybridoma Growth Media ISF-I
Supplier: BIOCONCEPT
ISF-1 is a serum-free, chemically defined medium for hybridoma cell culture and monoclonal antibody production.
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HyClone™ CDM4NS0 media
Supplier: HyClone products (Cytiva)
HyClone™ CDM4NS0 media was developed through Metabolic Pathway Design approach to increase process yields in the manufacture of monoclonal antibodies (MAbs) for therapeutic use using a variety of cholesterol auxotrophic NS0 cell clones.
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Murashige cattleya orchid multiplication medium
Supplier: Apollo Scientific
Contains the macro- and micronutrients as described by Murashige and Skoog (1962). Plant tissue culture tested.
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Terrestrial (Cypripedium) orchid medium
Supplier: Apollo Scientific
Contains 400 mg/L calcium nitrate and 400 mg/L Casein; WITHOUT ammonium nitrate.
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Vacin and Went modified orchid basal salt mixture
Supplier: Apollo Scientific
Contains an equivalent iron molar concentration of ferrous sulfate in place of ferric tartrate.
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Orchid multiplication medium without charcoal and agar
Supplier: Apollo Scientific
Plant tissue culture tested.
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Murashige and skoog modified basal medium (with 6-BAP)
Supplier: Apollo Scientific
Murashige and skoog modified basal medium (with 6-BAP) 10 L
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Murashige and skoog modified basal salts (½ Micros and ½ Macros)
Supplier: Apollo Scientific
Contains macro/micronutrients, sucrose, agar and organics as described by Murashige and Skoog, 1962 Plant tissue culture testedFull specification given in appendix.
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Smooth Muscle Cell Growth Medium, Basal
Supplier: PROVITRO
Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.
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hMSC Adipogenesis Induction Medium
Supplier: PROVITRO
The hMSC adipogenesis induction medium is a sterile liquid culture medium for inducing adipogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement kit components.
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CMRL 1066, supplemented, Corning®
Supplier: Corning
Corning®, CMRL 1066, supplemented, storage temperature: 2 to 8 °C
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RPMI 1640, Corning®
Supplier: Corning
Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol.
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Reduced-serum medium, Corning® transfectagro™
Supplier: Corning
Corning® transfectagro® Reduced Serum Medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation
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HyClone™ SFM4CHO media
Supplier: HyClone products (Cytiva)
HyClone™ SFM4CHO media is a serum-free and protein-free formulation that contains no components of bovine origin and has been designed for high performance in a variety of culture vessels, including bioreactors.
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Cell culture media, Dulbecco's Modified Eagles Medium (DMEM), Low Glucose, HyClone™
Supplier: HyClone products (Cytiva)
DMEM Low Glucose was the original modification of Basal Medium Eagle (BME) used to grow mouse embryonic cells. DMEM, Low Glucose is suitable for the culturing of many immortalised cell lines from humans, monkeys, hamsters, rats, mice, chicken and fish. DMEM contains four times more amino acids, vitamins and other supplementary components than Basal Medium Eagle (BME), the base media from which DMEM was derived. As DMEM contains no growth-promoting agents or proteins, supplementation with FBS is recommended for optimal performance.
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Media IDMEM W/GLN+HPS power, Corning®
Supplier: Corning
Corning®, Iscove’s Modified DMEM: with L-glutamine and 25 mM HEPES, without [beta]-thioglycerol, [beta]-mercaptoethanol, and sodium bicarbonate, Powder
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Minimum essential medium (MEM) alpha medium, Corning®
Supplier: Corning
Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.
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Serum-free/protein-free medium, 1X, Corning® insectagro®
Supplier: Corning
Corning® insectagro® Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells.
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Protein and serum free medium, PF medium, Corning®
Supplier: Corning
PF medium is optimised for the cultivation of Chinese Hamster Ovary (CHO) cells, along with many derivatives of this parent line PF medium is a proprietary serum-free and protein-free growth medium that does not contain any hormones or growth factors. It medium supports superior growth and viability over long term passages in both adherent and suspension culture, and is formulated without L-glutamine.
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Corning® Lymphocyte serum-free medium
Supplier: Corning
The serum-free medium is used to support the growth of T-cells activated with immobilized anti-CD3 antibody. Interleukin-2 is a necessary component for a successful culture. This formulation has been shown to work with the following cell types: CIK/DC-CIK cell lines, T-cells, CAR-T, TCR-T and TIL.
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HyClone™ DMEM/F12 1:1 Cell Culture Media
Supplier: HyClone products (Cytiva)
HyClone™ DMEM/F12 is an equal parts blend of DMEM and Ham’s F12 media that is widely used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. This formulation combines the high concentration of glucose, amino acids and vitamins from DMEM with the wide variety of other components available in Ham’s F-12.
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Hink's TNM-FH medium, Corning®
Supplier: Corning
Hink's TNM-FH medium is a modified Grace's formulation containing lactalbumin hydrolysate (LAH) and ultrafiltered yeastolate, and was originally developed for the optimal propagation of cabbage looper, Tricoplusi in cells.
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Cell culture media, Corning®
Supplier: Corning
A complete range of classical cell culture media are available.
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Cell culture media, powdered, Leibovitz's L-15 HyClone™
Supplier: HyClone products (Cytiva)
HyClone Leibovitz L-15 Medium is manufactured using ISO 9001:2000-certified processes
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Dulbecco's modified eagle medium limiting media (DMEM-LM)
Supplier: Thermo Fisher Scientific
Cell culture media, Limiting medium, DMEM (-)L-leucine, (-)L-methionine with 4,5g/l glucose, 4,0 mM L-glutamine, sodium pyruvate and phenol red, Liquid
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Medium, Ham's F-12, Corning®
Supplier: Corning
Ham's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids.