Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
Endothelial Cell Proliferations Medium
Supplier: PROVITRO
Endothelial cell proliferation medium is a sterile liquid culture medium for culturing endothelial cells. The medium is delivered as a basal medium and is suitable for culturing human endothelial cells after adding the supplement mix components.
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Microvascular Endothelial Cell Growth Medium, FCS
Supplier: PROVITRO
Microvascular Endothelial Cell Growth Medium, FCS, is a sterile liquid culture medium designed for cultivating human microvascular endothelial cells (HMVEC). Delivered as a basal medium, it is suitable for culturing Provitro HMVEC upon the addition of supplement mix components.
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hMSC Osteogenesis Induction Medium
Supplier: PROVITRO
The hMSC osteogenesis induction medium is a sterile liquid culture medium for inducing osteogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement mix components.
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Cell culture media, Medium 199
Supplier: BIOWEST
The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.
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HyCell™ CHO media, Hyclone™
Supplier: HyClone products (Cytiva)
HyCell™ CHO media has been developed for CHO cell culture for bioprocess applications.
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Tryptose Phosphate Broth, MP Biomedicals
Supplier: MP Biomedicals
Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
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BIT 9500 serum substitute
Supplier: BIOCONCEPT
Replaces FBS/FCS/serum in all cell culture media including various media for stem cells, primary cells and cell lines.
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HyClone™ SFM4HEK293, HEK 293 Media
Supplier: HyClone products (Cytiva)
HyClone™ SFM4HEK293 is a protein-free cell culture media without animal-derived components (ADCF) used to support production of adenoviral vectors and recombinant proteins in HEK 293 cultures.
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Cell culture media, CMRL 1066
Supplier: BIOWEST
CRML was originally formulated for promoting strain L (Earle) cells under serum-free conditions. These media are also known for its ability to sustain cloning of monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.
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Orchid maintenance/Replate medium with banana and charcoal, without agar
Supplier: Apollo Scientific
Orchid Maintenance/Replate Medium with Banana and Charcoal, without Agar 50 L
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Cell culture media, Ham's F14 (F-14 Nutrient Medium)
Supplier: BIOWEST
Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.
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Cape Sundew/Venus Flytrap multiplication basal medium
Supplier: Apollo Scientific
Cape Sundew/Venus Flytrap multiplication basal medium 10 L
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Lloyd and McCown's woody plant micronutrient mixture
Supplier: Apollo Scientific
Contains the WPM micronutrients described by Lloyd and McCown (1981). Plant tissue culture tested.
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Orchid maintence/replate medium contains banana, charcoal, and agar
Supplier: Apollo Scientific
Orchid maintence / replate medium contains banana, charcoal, and agar 50 L
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NLN medium
Supplier: Apollo Scientific
Plant tissue culture tested. Full specification given in appendix.
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Gamborg's basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Gamborget al. 1968. Plant tissue culture tested. Full specification given in appendix.
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Cell culture media, Schneider's Insect Medium
Supplier: BIOWEST
Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, Drosophila melanogaster. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.
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Cell culture media, Basal Medium Eagle (BME)
Supplier: BIOWEST
Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).
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Chu N6 basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as describedby Chu, 1975. Plant tissue culture testedFull specification given in appendix.
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Ichihashi new phalaenopsis (NP) medium
Supplier: Apollo Scientific
Contains the components as described by Ichihashi (1992); modified to contain 82,0 mg/L NH₄NO₃.
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Cell culture medium, HyClone™ ActiPro
Supplier: HyClone products (Cytiva)
HyClone™ ActiPro cell culture medium has been formulated to provide high yields of recombinant proteins in processes using Chinese hamster ovary (CHO) cell lines.
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Orchid seed sowing medium II
Supplier: Apollo Scientific
Mother flasking medium.
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Endothelial Cell Growth Medium, Advanced
Supplier: PROVITRO
Endothelial Cell Growth Medium, Advanced, is a sterile liquid culture medium designed for the cultivation of endothelial cells. Delivered in the form of a basal medium, it is well-suited for culturing Provitro human endothelial cells when supplemented with the provided mix components.
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Keratinocyte Growth Medium
Supplier: PROVITRO
Keratinocyte Growth Medium is a sterile liquid serum-free culture medium designed for cultivating human keratinocytes (HKER). Delivered as a basal medium, it is suitable for culturing Provitro HKER upon the addition of the supplement mix.
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Cell culture media, liquid, DMEM:F12 (1:1), HyClone™
Supplier: HyClone products (Cytiva)
HyClone Classical Liquid Media: DMEM F12 is manufactured using ISO 9001:2000-certified processes.
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HyClone™ Classical Liquid Media: RPMI 1640, HyClone products (Cytiva)
Supplier: HyClone products (Cytiva)
HyClone RPMI 1640 Media is manufactured using ISO 9001:2000-certified processes
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Kao and Michayluk basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients required to initiate plant growthPlant tissue culture testedFull specification given in appendix.
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Murashige and skoog modified basal salts (½ nitrogen)
Supplier: Apollo Scientific
Contains macro/micronutrients as described byMurashige and Skoog, 1962. Modified by reducing NH₄NO₃ and KNO₃ contentPlant tissue culture testedFull specification given in appendix.
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White's basal salt mixture
Supplier: Apollo Scientific
With the macro/micronutrients as described by White, 1968. Full specification given in appendix.
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Schenk and Hildebrandt basal salt mixture
Supplier: Apollo Scientific
Schenk and Hildebrandt basal salt mixture 10×1 L