Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
MethoCult™ M3231 Medium
Supplier: Stemcell Technologies
Methylcellulose-based medium without cytokines for mouse cells.
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GyneCult™ Fallopian Tube Organoid Medium
Supplier: Stemcell Technologies
Serum-free and sex steroid-modular kit for the expansion and differentiation of human fallopian tube organoids.
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N2 Supplement-B
Supplier: Stemcell Technologies
For neural differentiation of mouse and human ES and iPS cells.
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MethoCult™ SF M3236 Medium
Supplier: Stemcell Technologies
Serum-free methylcellulose-based medium without cytokines for mouse cells.
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NeuroCult™ SM1 Neuronal Supplement
Supplier: Stemcell Technologies
Supplement (50X) for the serum-free culture of neurons.
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StemSpan™ Lymphoid Differentiation Coating Material (100X)
Supplier: Stemcell Technologies
Coating material for the differentiation of lymphoid cells.
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MethoCult™-GF H84444 Medium
Supplier: Stemcell Technologies
Pre-aliquoted methylcellulose-based medium with recombinant cytokines. 24×3 ml vials.
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ClonaCell™-CHO CD Liquid Medium
Supplier: Stemcell Technologies
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free liquid medium for culture of CHO cells.
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RSeT™ Medium (2-Component)
Supplier: Stemcell Technologies
Defined medium for naïve-like human pluripotent stem cells.
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Passage Reagent Group™ Reagents
Supplier: CELL SYSTEMS
Optimize the plating, passaging and freezing of cell cultures with Cell Systems Reagents. Growth supplements and antibiotic products are also available to ensure each experiment is initiated in an optimal environment.
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ClonaCell™ TCS/Hygromycin Kit
Supplier: Stemcell Technologies
Kit containing ClonaCell™-TCS Medium and hygromycin B antibiotic.
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StemSpan™ Serum-Free Expansion Medium
Supplier: Stemcell Technologies
Serum-free medium for culture and expansion of hematopoietic cells.
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MethoCult™ GF H84435 Medium
Supplier: Stemcell Technologies
Methylcellulose-based medium with recombinant cytokines. 1×100 ml bottle.
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MethoCult™ GF H84434 Medium
Supplier: Stemcell Technologies
Methylcellulose-based medium with recombinant cytokines. 1×100 ml bottle.
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MethoCult™ GF H84445 Medium
Supplier: Stemcell Technologies
Pre-aliquoted methylcellulose-based medium with recombinant cytokines. 24×3 ml vials.
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L-Glutamine
Supplier: Stemcell Technologies
Cell culture supplement. 1×3 ml vial.
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Leverantör: VWR Chemicals
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