Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator
Supplier: Stemcell Technologies
cGMP, human T cell activation and expansion reagent.
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ImmunoCult™ Dendritic Cell Maturation Supplement
Supplier: Stemcell Technologies
Supplement for the maturation of human monocyte-derived dendritic cells.
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StemSpan-AOF Medium
Supplier: Stemcell Technologies
For culture and expansion of human hematopoietic cells - 500 ml.
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MyoCult™ Expansion 10X Supplement (Mouse)
Supplier: Stemcell Technologies
Serum-containing supplement for the expansion of mouse skeletal muscle progenitor cells (satellite cells).
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PancreaCult™ Organoid Growth Medium (Human)
Supplier: Stemcell Technologies
Culture medium kit for growth, and establishment of human pancreatic organoids.
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MesenCult™ SF Attachment Substrate
Supplier: Stemcell Technologies
Serum-free attachment substrate for human mesenchymal stem cells - 5 mg.
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ES-Cult™ M3120 Medium
Supplier: Stemcell Technologies
Base methylcellulose medium for in vitro differentiation of mouse ES or iPS cells to hematopoietic or endothelial progenitor cells.
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StemSpan™ CC110 Culture Supplement
Supplier: Stemcell Technologies
Serum-free culture supplement for expansion of human hematopoietic cells.
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BrainPhys™ Neuronal Medium
Supplier: Stemcell Technologies
Serum-free neurophysiological basal medium for improved neuronal function.
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StemSpan™-AOF Medium
Supplier: Stemcell Technologies
For culture and expansion of human hematopoietic cells.
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MethoCult™-GF H84444 Medium
Supplier: Stemcell Technologies
Pre-aliquoted methylcellulose-based medium with recombinant cytokines. 24×3 ml vials.
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ClonaCell™-CHO CD Liquid Medium
Supplier: Stemcell Technologies
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free liquid medium for culture of CHO cells.
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ClonaCell™ TCS/Hygromycin Kit
Supplier: Stemcell Technologies
Kit containing ClonaCell™-TCS Medium and hygromycin B antibiotic.
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N2 Supplement-A
Supplier: Stemcell Technologies
For neural and pancreatic differentiation of mouse and human ES and iPS cells.
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MesenCult™ Proliferation Kit (Human)
Supplier: Stemcell Technologies
Medium for detection of CFU-F and expansion of human mesenchymal stem cells.
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MethoCult™ H4535 Enriched without EPO
Supplier: Stemcell Technologies
Pre-aliquoted methylcellulose-based medium with recombinant cytokines (without EPO) for human cells. 24×3 ml vials.
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Catalog #: 20864.320
: VWR Chemicals
Catalog #: 83640.320
: VWR Chemicals
Catalog #: 31770.294
: VWR Chemicals
Catalog #: 1.00030.2500
: Merck
Catalog #: 83645.320
: VWR Chemicals
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: VWR Chemicals