Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
BalanCD™ CHO Feed 3, Powder
Supplier: Shenandoah Biotechnology
BalanCD™ CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode.
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HyClone™ Peak Expression Medium
Supplier: HyClone products (Cytiva)
Ready-to-use medium supports high cell growth, exceptional transfection efficiency, and robust viral production for adeno-associated virus and lentivirus expressed in human embryonic kidney (HEK293) cell lines.
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Endothelial Cell Proliferation Medium, Basal
Supplier: PROVITRO
Endothelial cell proliferation medium is a sterile liquid culture medium for culturing endothelial cells. The medium is delivered as a basal medium and is suitable for culturing human endothelial cells after adding the supplement mix components.
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Smooth Muscle Cell Growth Medium
Supplier: PROVITRO
Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.
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Gamborg's B-5 basal salt medium
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Gamborget al. 1968. Plant tissue culture tested. Full specification given in appendix.
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Fast terrestrial orchid medium
Supplier: Apollo Scientific
Contains sucrose, fructose and agar.
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Chu N6 basal salt medium with vitamins
Supplier: Apollo Scientific
Contains macro/micronutrients and vitamins as describedby Chu, 1975.Plant tissue culture testedFull specification given in appendix.
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Orchid Maintenance medium with charcoal, without agar
Supplier: Apollo Scientific
Orchid Maintenance Medium with Charcoal, without Agar 50 L
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Cell culture media, Ham's F10 (F-10 Nutrient Medium)
Supplier: BIOWEST
Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.
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Cell culture media, Liebovitz L-15 medium
Supplier: BIOWEST
The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.
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Vacin and went modified orchid medium with agar and sucrose
Supplier: Apollo Scientific
Contains the macro- and micronutrients as described by Vacin and Went (1949).
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Murashige fern multiplication medium
Supplier: Apollo Scientific
Murashige fern multiplication medium 50 L
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Murashige and skoog basal medium with vitamins
Supplier: Apollo Scientific
Murashige and skoog basal medium with vitamins 10×1 L
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Lloyd and McCown's woody plant basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Lloyd and McCown, 1981. Plant tissue culture tested. Full specification given in appendix.
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Cell culture media, Minimum Essential Medium Eagle (Modified), MP Biomedicals
Supplier: MP Biomedicals
Minimum Essential Medium (MEM) is a general use medium ideal for the growth and maintenance of a wide range of mammalian cell types. MEM with Earle's salts and L-glutamine has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle’s balanced salts, which makes it suitable for use in atmospheres charged with CO₂ gas.
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HyClone™ Liquid Cell Culture Media, Minimal Essential Medium (MEM), HyClone products (Cytiva)
Supplier: HyClone products (Cytiva)
Minimum Essential Medium (MEM) is one of the earliest modifications of Basal Medium Eagle (BME) containing amino acid concentrations that more closely mimic those found in mammalian cells. MEM has been used with serum supplementation on a broad range of cell types including suspension and adherent mammalian cells, HeLa, BHK-21, HEK-293, HEp-2, HT-1080, MCF-7,fibroblasts and primary rat astrocytes.
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Smooth Muscle Cell Growth Medium
Supplier: PROVITRO
Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.
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Anderson's Basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as described byAnderson, 1978/80 Plant tissue culture testedFull specification given in appendix.
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Murashige and skoog modified basal medium (with 2iP)
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Murashige and Skoog, 1962. Modified with addition of 2iP and Adenine hemisulphate. Plant tissue culture testedFull specification given in appendix.
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Malmgren modified terrestrial orchid medium (without sucrose, agar and pineapple powder)
Supplier: Apollo Scientific
Plant tissue culture tested.
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Murashige and skoog modified basal salts (contains no N, P or K)
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Murashige and Skoog, 1962. Modified by eliminating NH4NO3 and KNO3. Plant tissue culture tested. Full specification given in appendix.
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H1 oat medium
Supplier: Apollo Scientific
Contains agar and sucrose. Contains the macro- and micronutrients and supplements required for the symbiotic culture of orchids.
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Orchid multiplication medium with agar, without charcoal
Supplier: Apollo Scientific
Mother flasking medium III.
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Cell culture media, powdered, D-MEM, HyClone™
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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CHO cell culture platform media, HyClone™ SFM4CHO Utility
Supplier: HyClone products (Cytiva)
HyClone™ SFM4CHO-Utility media is ideally suited for the cost-effective manufacturing of recombinant proteins for academic and industrial research, genomics and proteomics, drug target screening and validation, and manufacturing of preclinical lots. It is formulated using a proprietary lipid complexing process for enhanced stability and broad-spectrum growth promotion.
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HyClone™ Ham's Nutrient Mixture F10/F12, Cell Culture Media, Powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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SF-4 baculo express insect medium
Supplier: BIOCONCEPT
SF-4 baculovirus expression medium allows high yield expression of proteins well above all other commercially available media. The products has no variability therefore does not require initial batch testing and batch reservation.
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CDM4Avian Media, HyClone™, HyClone products
Supplier: HyClone products (Cytiva)
Designed for vaccine manufacturing using suspension cell culture with avian cell line.
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Cell culture media, Schneider's Insect Medium
Supplier: BIOWEST
Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, Drosophila melanogaster. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.
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Cell culture media, Basal Medium Eagle (BME)
Supplier: BIOWEST
Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).