Cellodlingsmedium

Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:

• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.

Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.

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Orchid seed sowing medium

Supplier: Apollo Scientific

Mother flasking medium II.

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Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)

Supplier: BIOWEST

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

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SOB Medium, MP Biomedicals

Supplier: MP Biomedicals

SOB Medium rich medium for the preparation of competent host cells prior to transformation. Certified for plasmid and other bacterial cultures in liquid medium. Standard media for preparing cells for chemical transformation.

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HyClone AdvanceSTEM™ Stem Cell Media, Mesenchymal Stem Cell Expansion Kit, HyClone products (Cytiva)

Supplier: HyClone products (Cytiva)

AdvanceSTEM™ Mesenchymal Stem Cell Basal Medium and Mesenchymal Stem Cell Growth Supplement have been formulated to support expansion and maintenance of undifferentiated human mesenchymal stem cells (hMSCs).

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HyClone™ AdvanceSTEM cell culture media

Supplier: HyClone products (Cytiva)

HyClone™ AdvanceSTEM™ cell culture media are optimised for growth of embryonic stem cell cultures.

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Murashige and skoog modified basal salts (Macro-nutrient salt base-1X)

Supplier: Apollo Scientific

Murashige and skoog modified basal salts (macro-nutrient salt base-1X) 10×1 L

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Cell culture media, Dulbecco's Modified Eagles Medium (DMEM), High Glucose, HyClone™

Supplier: HyClone products (Cytiva)

HyClone™ DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture manufacturing processes.

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Smooth Muscle Cell Growth Medium

Supplier: PROVITRO

Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.

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BalanCD™ CHO Feed 3, Powder

Supplier: Shenandoah Biotechnology

BalanCD™ CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode.

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HyClone™ MEM (modified), Cell culture media, powdered

Supplier: HyClone products (Cytiva)

Dry powdered media for supporting cell culture growth and biomanufacturing processes.

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HyClone™ AdvanceSTEM ES Qualified Amino Acids, HyClone products

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified Amino Acids have been optimized for growth of embryonic cell cultures.

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BSK-H medium for the cultivation of Borrelia sp.

Supplier: BIOCONCEPT

BSK-H medium is a high quality nutrient liquid for the reliable cultivation of Borrelia sp.

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HyClone™ Peak Expression Medium

Supplier: HyClone products (Cytiva)

Ready-to-use medium supports high cell growth, exceptional transfection efficiency, and robust viral production for adeno-associated virus and lentivirus expressed in human embryonic kidney (HEK293) cell lines.

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CHO cell culture platform liquid media, HyClone™ HyCell™ TransFx-C

Supplier: HyClone products (Cytiva)

HyCell™ TransFx-C Media is a hydrolysate-free and regulatory-friendly media which supports transient transfection, growth, and expression of a variety of recombinant proteins in CHO cells.