Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
HyClone™ CDM4MAb Cell Culture Media
Supplier: HyClone products (Cytiva)
ADCF medium for use with hybridoma and myeloma cell lines.
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HyClone™ RPMI 1640, Cell Culture Media, Powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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HyClone™ Ham's F-12 Basal Media, Cell Culture Media, Liquid
Supplier: HyClone products (Cytiva)
Ham's F-12 media was originally derived by modifying Ham's F-10 to include increased concentrations of choline, inositol, putrescine, and several amino acids. It was developed specifically for serum free culturing of Chinese Hamster Ovary (CHO) cells and has also been used with supplementation to grow chondrocytes, primary rat hepatocytes, rat prostate epithelial cells, carcinoma cells, rat skeletal myoblasts and rat, rabbit, and chicken embryos.
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Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)
Supplier: BIOWEST
In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.
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HyClone™ MEM (modified), Cell culture media, powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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HyClone AdvanceSTEM™ Stem Cell Media, Mesenchymal Stem Cell Expansion Kit, HyClone products (Cytiva)
Supplier: HyClone products (Cytiva)
AdvanceSTEM™ Mesenchymal Stem Cell Basal Medium and Mesenchymal Stem Cell Growth Supplement have been formulated to support expansion and maintenance of undifferentiated human mesenchymal stem cells (hMSCs).
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Cell culture media, Dulbecco's Modified Eagles Medium (DMEM), High Glucose, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture manufacturing processes.
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HyClone™ Classical Liquid Media: Medium 199, HyClone products (Cytiva)
Supplier: HyClone products (Cytiva)
HyClone Medium 199 is manufactured using ISO 9001:2000-certified processes
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HyClone™ AdvanceSTEM cell culture media
Supplier: HyClone products (Cytiva)
HyClone™ AdvanceSTEM™ cell culture media are optimised for growth of embryonic stem cell cultures.
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HyClone™ AdvanceSTEM ES Qualified Amino Acids, HyClone products
Supplier: HyClone products (Cytiva)
HyClone AdvanceSTEM ES Qualified Amino Acids have been optimized for growth of embryonic cell cultures.
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HyClone™ CDM4HEK293
Supplier: HyClone products (Cytiva)
HyClone™ CDM4HEK293 media are chemically-defined, animal-derived component free (ADCF) cell culture media.
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SOB Medium, MP Biomedicals
Supplier: MP Biomedicals
SOB Medium rich medium for the preparation of competent host cells prior to transformation. Certified for plasmid and other bacterial cultures in liquid medium. Standard media for preparing cells for chemical transformation.
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CHO cell culture platform liquid media, HyClone™ HyCell™ TransFx-C
Supplier: HyClone products (Cytiva)
HyCell™ TransFx-C Media is a hydrolysate-free and regulatory-friendly media which supports transient transfection, growth, and expression of a variety of recombinant proteins in CHO cells.
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Murashige and skoog modified basal salts (Macro-nutrient salt base-1X)
Supplier: Apollo Scientific
Murashige and skoog modified basal salts (macro-nutrient salt base-1X) 10×1 L
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BSK-H medium for the cultivation of Borrelia sp.
Supplier: BIOCONCEPT
BSK-H medium is a high quality nutrient liquid for the reliable cultivation of Borrelia sp.
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BalanCD™ CHO Feed 3, Powder
Supplier: Shenandoah Biotechnology
BalanCD™ CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode.
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HyClone™ Peak Expression Medium
Supplier: HyClone products (Cytiva)
Ready-to-use medium supports high cell growth, exceptional transfection efficiency, and robust viral production for adeno-associated virus and lentivirus expressed in human embryonic kidney (HEK293) cell lines.
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Endothelial Cell Proliferation Medium, Basal
Supplier: PROVITRO
Endothelial cell proliferation medium is a sterile liquid culture medium for culturing endothelial cells. The medium is delivered as a basal medium and is suitable for culturing human endothelial cells after adding the supplement mix components.
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Smooth Muscle Cell Growth Medium
Supplier: PROVITRO
Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.
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Gamborg's B-5 basal salt medium
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Gamborget al. 1968. Plant tissue culture tested. Full specification given in appendix.
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Fast terrestrial orchid medium
Supplier: Apollo Scientific
Contains sucrose, fructose and agar.
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Chu N6 basal salt medium with vitamins
Supplier: Apollo Scientific
Contains macro/micronutrients and vitamins as describedby Chu, 1975.Plant tissue culture testedFull specification given in appendix.
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Orchid Maintenance medium with charcoal, without agar
Supplier: Apollo Scientific
Orchid Maintenance Medium with Charcoal, without Agar 50 L
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Cell culture media, Ham's F10 (F-10 Nutrient Medium)
Supplier: BIOWEST
Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.
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Cell culture media, Liebovitz L-15 medium
Supplier: BIOWEST
The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.
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Smooth Muscle Cell Growth Medium
Supplier: PROVITRO
Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.
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HyClone™ Liquid Cell Culture Media, Minimal Essential Medium (MEM), HyClone products (Cytiva)
Supplier: HyClone products (Cytiva)
Minimum Essential Medium (MEM) is one of the earliest modifications of Basal Medium Eagle (BME) containing amino acid concentrations that more closely mimic those found in mammalian cells. MEM has been used with serum supplementation on a broad range of cell types including suspension and adherent mammalian cells, HeLa, BHK-21, HEK-293, HEp-2, HT-1080, MCF-7,fibroblasts and primary rat astrocytes.
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Cell culture media, powdered, D-MEM, HyClone™
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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CHO cell culture platform media, HyClone™ SFM4CHO Utility
Supplier: HyClone products (Cytiva)
HyClone™ SFM4CHO-Utility media is ideally suited for the cost-effective manufacturing of recombinant proteins for academic and industrial research, genomics and proteomics, drug target screening and validation, and manufacturing of preclinical lots. It is formulated using a proprietary lipid complexing process for enhanced stability and broad-spectrum growth promotion.
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HyClone™ Ham's Nutrient Mixture F10/F12, Cell Culture Media, Powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.