Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
Cell culture media, Glasgow MEM (GMEM BHK 21)
Supplier: BIOWEST
Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.
Expand 3 Items
DMEM Low Glucose with Stable Glutamine with Sodium Pyruvate
Supplier: BIOWEST
Dulbecco's Modified Eagle medium (DMEM) is formulated to preserve and maintain the growth of a wide range of mammalian cells. Supplemented with fetal calf serum, DMEM also allows the proliferation of mouse embryonic stem cells.
Expand 1 Items
Cell culture media, McCoy's 5A medium
Supplier: BIOWEST
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
Expand 4 Items
HyClone SFMTranfx-293-medium
Supplier: HyClone products (Cytiva)
HyClone™ SFMTransfx-293 är ett serumfritt medium, fritt från animaliskt deriverade komponenter, som är utformat för att stödja tillväxten av HEK 293-odlingar och gynna transfektion med hjälp av lipofektion eller liknande metoder. Tillverkat enligt GMP-riktlinjer.
Expand 2 Items
Phytotech Orchid replate medium
Supplier: Apollo Scientific
Proprietary formulation.
Expand 2 Items
Hybridoma growth and expression CD media HYGM7
Supplier: BIOCONCEPT
Hybridoma growth media (HYGM) 7 is a serum-free, protein free, chemically defined (CD) and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.
Expand 3 Items
Terrestrial (Cypripedium) orchid medium, contains 600 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 600 mg/L calcium nitrate and 200 mg/L casein; without ammonium nitrate.
Expand 2 Items
Skeletal Muscle Cell Growth Medium
Supplier: PROVITRO
Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.
Expand 2 Items
NB basal medium
Supplier: Apollo Scientific
With the macronutrients as described by Chu (1975) andthe micronutrients and vitamins as described by Gamborg et. al., 1968 Plant tissue culture testedFull specification given in appendix.
Expand 1 Items
Cell culture media, liquid, Iscove's Modified Dulbecco's Medium (IMDM), HyClone™
Supplier: HyClone products (Cytiva)
HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.
Expand 5 Items
Murashige shoot tip rooting medium
Supplier: Apollo Scientific
Contains macro/micronutrients, sucrose, agar andorganics as described by Malmgren, 1996 Plant tissue culture testedFull specification given in appendix.
Expand 1 Items
Musa (banana) multiplication medium
Supplier: Apollo Scientific
IITA Formulation. Plant tissue culture tested.
Expand 2 Items
Murashige and skoog Medium with vitamins and sucrose
Supplier: Apollo Scientific
Modified version of product number PMM519, contains 30 g/L D-Sucrose. Plant Tissue Culture Tested. Soluble in water.
Expand 2 Items
Murashige and skoog modified basal salts (without NH₄NO₃)
Supplier: Apollo Scientific
Murashige and Skoog Modified Basal Salts (without NH4NO3) 50 L
Expand 2 Items
HyClone™ SFM4MAb media
Supplier: HyClone products (Cytiva)
HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.
Expand 1 Items
Hyclone™ Ham's F-10 Basal Media, Cell Culture Media, Liquid, Cytiva
Supplier: HyClone products (Cytiva)
Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.
Expand 1 Items
Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".
Otherwise, you will receive generic recommendations.



