Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
Cell culture medium, ActiSM, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ ActiSM cell culture media is a chemically defined animal-derived component-free (ADCF) cultured media optimised for high-yield protein production for bioprocessing. This media is designed for use with HyClone™ ActiPro cell culture media.
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Cell culture media, Glasgow MEM (GMEM BHK 21)
Supplier: BIOWEST
Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.
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HyClone™ CDM4CHO, CHO Cell Culture Platform Liquid Media
Supplier: HyClone products (Cytiva)
HyQ® CDM4CHO™ is a chemically-defined protein-free medium containing no animal derived components. It is designed to increase the process yields for the industrial manufacture of recombinant proteins using a variety of CHO cell clones. HyQ® CDM4CHO™ has been successfully tested in various culture systems, including T-flasks, shaker flasks and bioreactors. HyQ® CDM4CHO™ contains Pluronic® F68 and L-glutamine, and does not contain phenol red.
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Cell culture media, HyClone™ ActiPro™
Supplier: HyClone products (Cytiva)
HyClone™ ActiPro™ cell culture media has been formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines. The cultured medium is chemically defined, animal-derived component-free (ADCF), and optimized for high-yield protein expression and production in batch or CHO fed-batch cell culture processes.
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Murashige and skoog basal mixture
Supplier: Apollo Scientific
Murashige and skoog basal mixture 10 L
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Knudson C orchid medium
Supplier: Apollo Scientific
Knudson C orchid medium 10 L
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Nitsch and Nitsch basal salt mixture
Supplier: Apollo Scientific
With the macro/micronutrients as described by Nitsch and Nitsch, 1969 Plant tissue culture tested Full specification given in appendix.
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Orchid seed sowing medium
Supplier: Apollo Scientific
Mother flasking medium II.
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Quoirin and Lepoivre basal salt mixture
Supplier: Apollo Scientific
Quoirin and Lepoivre basal salt mixture 10×1 L
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Heller's basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Heller, 1953 Plant tissue culture testedFull specification given in appendix.
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Terrestrial (Cypripedium) orchid medium, contains 400 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 400 mg/L calcium nitrate, without casein. Mother flasking medium V.
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DMEM Low Glucose with Stable Glutamine with Sodium Pyruvate
Supplier: BIOWEST
Dulbecco's Modified Eagle medium (DMEM) is formulated to preserve and maintain the growth of a wide range of mammalian cells. Supplemented with fetal calf serum, DMEM also allows the proliferation of mouse embryonic stem cells.
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Fibroblast Growth Medium, Basal
Supplier: PROVITRO
Fibroblast Growth Medium is a sterile liquid culture medium designed for cultivating human fibroblasts (HFIB). Provided as a basal medium, it is ideal for culturing Provitro HFIB upon the addition of supplement mix components.
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hMSC Chondrogenesis Induction Medium
Supplier: PROVITRO
The hMSC Chondrogenesis Induction Medium is a sterile liquid culture medium specifically designed for initiating chondrogenic differentiation in human mesenchymal stem cells (hMSCs). This medium is provided as a basal formulation and becomes fully functional for hMSC culture upon the addition of all the required supplement mix components.
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Microvascular Endothelial Cell Growth Medium, Basal
Supplier: PROVITRO
Microvascular Endothelial Cell Growth Medium, Advanced, is a sterile liquid culture medium designed for cultivating human microvascular endothelial cells (HMVEC). Delivered as a basal medium, it is suitable for culturing Provitro HMVEC upon the addition of supplement mix components.
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Urothelial Cell Growth Medium
Supplier: PROVITRO
Urothelial Cell Growth Medium is a sterile, serum-free liquid culture medium designed for the cultivation of Human Urothelial Epithelial Cells (HUEPC). Delivered in the form of a basal medium, it becomes a complete and suitable environment for culturing HUEPC upon the addition of three supplement components.
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HyClone™ VaccineXpress Cell Culture Media
Supplier: HyClone products (Cytiva)
HyClone VaccineXpress cell culture medium is designed and developed for high‑density growth and maintenance of kidney‑derived cell lines (e.g., Vero cells) for viral vaccine development. VaccineXpress medium is well suited for use in multi-well plates and large‑scale manufacturing using microcarriers in WAVE Bioreactor or Xcellerex bioreactors.
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Murashige and skoog modified basal salts (without KH₂PO₄)
Supplier: Apollo Scientific
Contains macro/micronutrients, sucrose, agar and organics as described by Murashige and Skoog, 1962.Plant tissue culture testedFull specification given in appendix.
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Malmgren modified terrestrial orchid medium
Supplier: Apollo Scientific
Malmgren modified terrestrial orchid medium 10×1 L
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Murashige and skoog modified basal medium (with 2iP And IAA)
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Murashige and Skoog, 1962. Modified with addition of 2iP, Adenine hemisulphate and IAA. Plant tissue culture testedFull specification given in appendix.
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HyClone™ IMDM, Cell culture media, powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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Insect cell culture media, serum-free, CCM3, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ CCM3 media is designed to increase cell yields with multiple lines in a variety of culture environments. It is tailored for Sf9 cells.
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Cell culture media, Minimum Essential Medium (MEM)
Supplier: BIOWEST
Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.
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Cell culture media, Ham's F12 (F-12 Nutrient Medium)
Supplier: BIOWEST
Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.
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Skeletal Muscle Cell Growth Medium
Supplier: PROVITRO
Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.
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hMSC Proliferation Medium, Basal
Supplier: PROVITRO
The hMSC Proliferation Medium is a sterile liquid culture medium designed for cultivating human mesenchymal stem cells (hMSC). Delivered as a basal medium, it is well-suited for culturing hMSC upon the addition of supplement mix components.
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HyClone™ Classical Liquid Media, McCoy's 5A, HyClone products (Cytiva)
Supplier: HyClone products (Cytiva)
HyClone Classical Liquid Media: McCoy's 5A is manufactured using ISO 9001:2000-certified processes.
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HyClone™ ADCF-mAb media
Supplier: HyClone products (Cytiva)
Protein-free and animal-derived component-free medium
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CHO cell culture platform liquid media, HyClone™ PF-CHO LS and PF-CHO MPS
Supplier: HyClone products (Cytiva)
HyClone™ PF-CHO LS and PF-CHO MPS media are designed to support the DHFR selection/amplification system. They have been successfully tested in a variety of cell culture systems, including T-flasks, spinner flasks, and bioreactors. PF-CHO MPS media is the powder version of PF-CHO LS media.