Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
HyClone SFX-Insect, cellodlingsmedium
Supplier: HyClone products (Cytiva)
HyClone™ SFX-Insect™ med L-glutamin är ett mångsidigt proteinfritt cellodlingsmedium som utvecklats genom design av metabola vägar för att stödja tillväxten av flera insektscellinjer och produktion av en mängd olika rekombinanta proteiner. Det ger överlägsen tillväxt av många nyckelinsektscellinjer, inklusive Sf9, Sf21 och High Five (T.ni), som kräver minimal anpassning.
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HyClone CDM4PERMAb
Supplier: HyClone products (Cytiva)
HyClone™ CDM4PERMAb är ett cellodlingsmedium med hög prestanda utvecklat för att öka processutbytet vid produktion av humana antikroppar och rekombinanta proteiner med hjälp av PER.C6-teknik (Crucell NV). Tillverkat enligt GMP-riktlinjer.
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Flytande cellodlingsmedium, McCoy's, BioWhittaker™
Supplier: LONZA
Kemiskt definierat flytande medium används för att tillföra näringsämnen för cellodling i tillämpningar inom forskning, diagnostik och tillverkning. Alla BioWhittaker™ klassiska mediaprodukter tillverkas i enlighet med cGMP-bestämmelser.
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Flytande cellodlingsmedium, Dulbecco's Modified Eagle's Medium (D-MEM), BioWhittaker™
Supplier: LONZA
Kemiskt definierat flytande medium används för att tillföra näringsämnen för cellodling i tillämpningar inom forskning, diagnostik och tillverkning. Alla BioWhittaker™ klassiska mediaprodukter tillverkas i enlighet med cGMP-bestämmelser.
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VWR®, Cell culture media, Minimum Essential Medium (MEM)
Supplier: VWR Chemicals
Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.
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VWR®, Bovine serum albumin (BSA)
Supplier: VWR Chemicals
VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.
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VWR®, L-Glutamine
Supplier: VWR Chemicals
L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.
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VWR®, Cell culture media, McCoy's 5A medium
Supplier: VWR Chemicals
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
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VWR®, Cell culture media, Ham's F12
Supplier: VWR Chemicals
Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.
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VWR®, Cell culture media, Schneider's Drosophila medium
Supplier: VWR Chemicals
Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.
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VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)
Supplier: VWR Chemicals
Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.
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VWR®, Lymphocyte separation media
Supplier: VWR Chemicals
Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.
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Cell culture media, McCoy's 5A medium
Supplier: BIOWEST
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
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Hyclone™ Ham's F-10 Basal Media, Cell Culture Media, Liquid, Cytiva
Supplier: HyClone products (Cytiva)
Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.
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Cell culture media, liquid, Iscove's Modified Dulbecco's Medium (IMDM), HyClone™
Supplier: HyClone products (Cytiva)
HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.
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Skeletal Muscle Cell Growth Medium
Supplier: PROVITRO
Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.
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Hybridoma growth and expression CD media HYGM7
Supplier: BIOCONCEPT
Hybridoma growth media (HYGM) 7 is a serum-free, protein free, chemically defined (CD) and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.
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HyClone SFMTranfx-293-medium
Supplier: HyClone products (Cytiva)
HyClone™ SFMTransfx-293 är ett serumfritt medium, fritt från animaliskt deriverade komponenter, som är utformat för att stödja tillväxten av HEK 293-odlingar och gynna transfektion med hjälp av lipofektion eller liknande metoder. Tillverkat enligt GMP-riktlinjer.
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LB Agar, Lennox Large Capsules, MP Biomedicals
Supplier: MP Biomedicals
Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
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Terrestrial (Cypripedium) orchid medium, contains 600 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 600 mg/L calcium nitrate and 200 mg/L casein; without ammonium nitrate.
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HyClone™ SFM4MAb media
Supplier: HyClone products (Cytiva)
HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.
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Musa (banana) multiplication medium
Supplier: Apollo Scientific
IITA Formulation. Plant tissue culture tested.
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Murashige shoot tip rooting medium
Supplier: Apollo Scientific
Contains macro/micronutrients, sucrose, agar andorganics as described by Malmgren, 1996 Plant tissue culture testedFull specification given in appendix.
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NB basal medium
Supplier: Apollo Scientific
With the macronutrients as described by Chu (1975) andthe micronutrients and vitamins as described by Gamborg et. al., 1968 Plant tissue culture testedFull specification given in appendix.
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Murashige and skoog modified basal salts (without NH₄NO₃)
Supplier: Apollo Scientific
Murashige and Skoog modified basal salts (without NH4NO3) 10×1 L
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Phytotech Orchid replate medium
Supplier: Apollo Scientific
Proprietary formulation.
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Murashige and skoog Medium with vitamins and sucrose
Supplier: Apollo Scientific
Modified version of product number PMM519, contains 30 g/L D-Sucrose. Plant Tissue Culture Tested. Soluble in water.
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VWR®, Cell culture media, RPMI 1640
Supplier: VWR Chemicals
RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.
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VWR®, Penicillin-streptomycin solution (100X)
Supplier: VWR Chemicals
VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.
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VWR®, Cell culture media, Medium 199
Supplier: VWR Chemicals
Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.