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Cellodlingsmedium

Cellodlingsmedium

Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:

• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.

Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.

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VWR®, Bovine serum albumin (BSA)

VWR®, Bovine serum albumin (BSA)

Supplier: VWR Chemicals

VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.

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VWR®, Cell culture media, Minimum Essential Medium (MEM)

VWR®, Cell culture media, Minimum Essential Medium (MEM)

Supplier: VWR Chemicals

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.

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VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)

VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)

Supplier: VWR Chemicals

Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

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VWR®, Lymphocyte separation media

VWR®, Lymphocyte separation media

Supplier: VWR Chemicals

Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

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VWR®, Cell culture media, Schneider's Drosophila medium

VWR®, Cell culture media, Schneider's Drosophila medium

Supplier: VWR Chemicals

Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.

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VWR®, Penicillin-streptomycin solution (100X)

VWR®, Penicillin-streptomycin solution (100X)

Supplier: VWR Chemicals

VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.

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VWR®, Cell culture media, Medium 199

VWR®, Cell culture media, Medium 199

Supplier: VWR Chemicals

Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

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VWR®, Cell culture media, RPMI 1640

VWR®, Cell culture media, RPMI 1640

Supplier: VWR Chemicals

RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.

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VWR®, Cell culture media, Ham's F12

VWR®, Cell culture media, Ham's F12

Supplier: VWR Chemicals

Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.

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VWR®, Cell culture media, McCoy's 5A medium

VWR®, Cell culture media, McCoy's 5A medium

Supplier: VWR Chemicals

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

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VWR®, L-Glutamine

VWR®, L-Glutamine

Supplier: VWR Chemicals

L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.

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Cell culture media, McCoy's 5A medium

Cell culture media, McCoy's 5A medium

Supplier: BIOWEST

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

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HyClone™ SFM4MAb media

HyClone™ SFM4MAb media

Supplier: HyClone products (Cytiva)

HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.

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Cell culture media, liquid, Iscove's Modified Dulbecco's Medium (IMDM), HyClone™

Cell culture media, liquid, Iscove's Modified Dulbecco's Medium (IMDM), HyClone™

Supplier: HyClone products (Cytiva)

HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.

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Skeletal Muscle Cell Growth Medium

Skeletal Muscle Cell Growth Medium

Supplier: PROVITRO

Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.

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Terrestrial (Cypripedium) orchid medium, contains 600 mg/L calcium nitrate

Supplier: Apollo Scientific

Contains 600 mg/L calcium nitrate and 200 mg/L casein; without ammonium nitrate.

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