Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
NLN medium
Supplier: Apollo Scientific
Plant tissue culture tested. Full specification given in appendix.
Expand 3 Items
Cell culture media, Ham's F14 (F-14 Nutrient Medium)
Supplier: BIOWEST
Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.
Expand 1 Items
Tryptose Phosphate Broth, MP Biomedicals
Supplier: MP Biomedicals
Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Expand 1 Items
Cell culture media, McCoy's 5A medium
Supplier: BIOWEST
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
Expand 3 Items
NB basal medium
Supplier: Apollo Scientific
With the macronutrients as described by Chu (1975) andthe micronutrients and vitamins as described by Gamborg et. al., 1968 Plant tissue culture testedFull specification given in appendix.
Expand 3 Items
Hybridoma growth and expression CD media HYGM7
Supplier: BIOCONCEPT
Hybridoma growth media (HYGM) 7 is a serum-free, protein free, chemically defined (CD) and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.
Expand 3 Items
HyClone SFMTranfx-293-medium
Supplier: HyClone products (Cytiva)
HyClone™ SFMTransfx-293 är ett serumfritt medium, fritt från animaliskt deriverade komponenter, som är utformat för att stödja tillväxten av HEK 293-odlingar och gynna transfektion med hjälp av lipofektion eller liknande metoder. Tillverkat enligt GMP-riktlinjer.
Expand 4 Items
Hyclone™ Ham's F-10 Basal Media, Cell Culture Media, Liquid, Cytiva
Supplier: HyClone products (Cytiva)
Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.
Expand 1 Items
Cell culture media, liquid, Iscove's Modified Dulbecco's Medium (IMDM), HyClone™
Supplier: HyClone products (Cytiva)
HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.
Expand 5 Items
HyClone™ SFM4MAb media
Supplier: HyClone products (Cytiva)
HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.
Expand 2 Items
Skeletal Muscle Cell Growth Medium
Supplier: PROVITRO
Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.
Expand 2 Items
Phytotech Orchid replate medium
Supplier: Apollo Scientific
Proprietary formulation.
Expand 3 Items
Murashige shoot tip rooting medium
Supplier: Apollo Scientific
Contains macro/micronutrients, sucrose, agar andorganics as described by Malmgren, 1996 Plant tissue culture testedFull specification given in appendix.
Expand 3 Items
Musa (banana) multiplication medium
Supplier: Apollo Scientific
IITA Formulation. Plant tissue culture tested.
Expand 3 Items
Murashige and skoog Medium with vitamins and sucrose
Supplier: Apollo Scientific
Modified version of product number PMM519, contains 30 g/L D-Sucrose. Plant Tissue Culture Tested. Soluble in water.
Expand 3 Items
Terrestrial (Cypripedium) orchid medium, contains 600 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 600 mg/L calcium nitrate and 200 mg/L casein; without ammonium nitrate.
Expand 3 Items
Murashige and skoog modified basal salts (without NH₄NO₃)
Supplier: Apollo Scientific
Murashige and Skoog modified basal salts (without NH4NO3) 10×1 L
Expand 2 Items
LB Agar, Lennox Large Capsules, MP Biomedicals
Supplier: MP Biomedicals
Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
Expand 1 Items
Cell culture medium, ActiSM, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ ActiSM cell culture media is a chemically defined animal-derived component-free (ADCF) cultured media optimised for high-yield protein production for bioprocessing. This media is designed for use with HyClone™ ActiPro cell culture media.
Expand 2 Items
Cell culture media, Glasgow MEM (GMEM BHK 21)
Supplier: BIOWEST
Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.
Expand 3 Items
HyClone™ CDM4CHO, CHO Cell Culture Platform Liquid Media
Supplier: HyClone products (Cytiva)
HyQ® CDM4CHO™ is a chemically-defined protein-free medium containing no animal derived components. It is designed to increase the process yields for the industrial manufacture of recombinant proteins using a variety of CHO cell clones. HyQ® CDM4CHO™ has been successfully tested in various culture systems, including T-flasks, shaker flasks and bioreactors. HyQ® CDM4CHO™ contains Pluronic® F68 and L-glutamine, and does not contain phenol red.
Expand 3 Items
Cell culture media, HyClone™ ActiPro™
Supplier: HyClone products (Cytiva)
HyClone™ ActiPro™ cell culture media has been formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines. The cultured medium is chemically defined, animal-derived component-free (ADCF), and optimized for high-yield protein expression and production in batch or CHO fed-batch cell culture processes.
Expand 2 Items
Murashige and skoog basal mixture
Supplier: Apollo Scientific
Murashige and skoog basal mixture 10 L
Expand 4 Items
Knudson C orchid medium
Supplier: Apollo Scientific
Knudson C orchid medium 10 L
Expand 3 Items
Nitsch and Nitsch basal salt mixture
Supplier: Apollo Scientific
With the macro/micronutrients as described by Nitsch and Nitsch, 1969 Plant tissue culture tested Full specification given in appendix.
Expand 2 Items
Orchid seed sowing medium
Supplier: Apollo Scientific
Mother flasking medium II.
Expand 3 Items
Quoirin and Lepoivre basal salt mixture
Supplier: Apollo Scientific
Quoirin and Lepoivre basal salt mixture 10×1 L
Expand 3 Items
Heller's basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Heller, 1953 Plant tissue culture testedFull specification given in appendix.
Expand 3 Items
Terrestrial (Cypripedium) orchid medium, contains 400 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 400 mg/L calcium nitrate, without casein. Mother flasking medium V.