Du sökte efter: Cellodlingsmedium
Cellodling är en av huvudteknikerna inom biovetenskap. Det är den allmänna termen som används för att avlägsna celler, vävnader eller organ från ett djur eller en växt och deras efterföljande placering i en konstgjord miljö som främjar deras överlevnad och proliferation. Det viktigaste och avgörande steget i cellodling är att välja lämpligt tillväxtmedium (cellodlingsmedium) för in vitro-odling. Det finns olika typer av cellmedia tillgängliga:
• Naturliga medier: består endast av naturligt förekommande biologiska vätskor.
• Syntetiska medier (artificial media): med tillsats av näringsämnen (både organiska och oorganiska), vitaminer, salter, O2- och CO2-gasfaser, serumproteiner, kolhydrater, cofaktorer.
Syntetiska medier grupperas i serum innehållande media med fetalt bovint serum (FBS) som vanligast tillsats, serumfria medier utan FBS, kemiskt definierade medier och proteinfria medier. VWR har media för olika celltyper, såsom för humana cellinjer, hybridomceller eller ovarieceller från kinesisk hamster.
HyClone SFX-Insect, cellodlingsmedium
Supplier: HyClone products (Cytiva)
HyClone™ SFX-Insect™ med L-glutamin är ett mångsidigt proteinfritt cellodlingsmedium som utvecklats genom design av metabola vägar för att stödja tillväxten av flera insektscellinjer och produktion av en mängd olika rekombinanta proteiner. Det ger överlägsen tillväxt av många nyckelinsektscellinjer, inklusive Sf9, Sf21 och High Five (T.ni), som kräver minimal anpassning.
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HyClone CDM4PERMAb
Supplier: HyClone products (Cytiva)
HyClone™ CDM4PERMAb är ett cellodlingsmedium med hög prestanda utvecklat för att öka processutbytet vid produktion av humana antikroppar och rekombinanta proteiner med hjälp av PER.C6-teknik (Crucell NV). Tillverkat enligt GMP-riktlinjer.
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Flytande cellodlingsmedium, McCoy's, BioWhittaker™
Supplier: LONZA
Kemiskt definierat flytande medium används för att tillföra näringsämnen för cellodling i tillämpningar inom forskning, diagnostik och tillverkning. Alla BioWhittaker™ klassiska mediaprodukter tillverkas i enlighet med cGMP-bestämmelser.
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Flytande cellodlingsmedium, Dulbecco's Modified Eagle's Medium (D-MEM), BioWhittaker™
Supplier: LONZA
Kemiskt definierat flytande medium används för att tillföra näringsämnen för cellodling i tillämpningar inom forskning, diagnostik och tillverkning. Alla BioWhittaker™ klassiska mediaprodukter tillverkas i enlighet med cGMP-bestämmelser.
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VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)
Supplier: VWR Chemicals
Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.
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VWR®, Cell culture media, Schneider's Drosophila medium
Supplier: VWR Chemicals
Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.
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VWR®, Lymphocyte separation media
Supplier: VWR Chemicals
Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.
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VWR®, Cell culture media, RPMI 1640
Supplier: VWR Chemicals
RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.
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VWR®, Penicillin-streptomycin solution (100X)
Supplier: VWR Chemicals
VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.
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VWR®, Cell culture media, Medium 199
Supplier: VWR Chemicals
Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.
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VWR®, Bovine serum albumin (BSA)
Supplier: VWR Chemicals
VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.
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VWR®, Cell culture media, Minimum Essential Medium (MEM)
Supplier: VWR Chemicals
Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.
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VWR®, Cell culture media, Ham's F12
Supplier: VWR Chemicals
Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.
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VWR®, L-Glutamine
Supplier: VWR Chemicals
L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.
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VWR®, Cell culture media, McCoy's 5A medium
Supplier: VWR Chemicals
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
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Cell culture medium, ActiSM, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ ActiSM cell culture media is a chemically defined animal-derived component-free (ADCF) cultured media optimised for high-yield protein production for bioprocessing. This media is designed for use with HyClone™ ActiPro cell culture media.
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Cell culture media, Glasgow MEM (GMEM BHK 21)
Supplier: BIOWEST
Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.
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HyClone™ CDM4CHO, CHO Cell Culture Platform Liquid Media
Supplier: HyClone products (Cytiva)
HyQ® CDM4CHO™ is a chemically-defined protein-free medium containing no animal derived components. It is designed to increase the process yields for the industrial manufacture of recombinant proteins using a variety of CHO cell clones. HyQ® CDM4CHO™ has been successfully tested in various culture systems, including T-flasks, shaker flasks and bioreactors. HyQ® CDM4CHO™ contains Pluronic® F68 and L-glutamine, and does not contain phenol red.
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Cell culture media, HyClone™ ActiPro™
Supplier: HyClone products (Cytiva)
HyClone™ ActiPro™ cell culture media has been formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines. The cultured medium is chemically defined, animal-derived component-free (ADCF), and optimized for high-yield protein expression and production in batch or CHO fed-batch cell culture processes.
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Murashige and skoog basal mixture
Supplier: Apollo Scientific
Murashige and skoog basal mixture 10 L
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Knudson C orchid medium
Supplier: Apollo Scientific
Knudson C orchid medium 10 L
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Nitsch and Nitsch basal salt mixture
Supplier: Apollo Scientific
With the macro/micronutrients as described by Nitsch and Nitsch, 1969 Plant tissue culture tested Full specification given in appendix.
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Orchid seed sowing medium
Supplier: Apollo Scientific
Mother flasking medium II.
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Quoirin and Lepoivre basal salt mixture
Supplier: Apollo Scientific
Quoirin and Lepoivre basal salt mixture 10×1 L
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Heller's basal salt mixture
Supplier: Apollo Scientific
Contains macro/micronutrients as described by Heller, 1953 Plant tissue culture testedFull specification given in appendix.
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Terrestrial (Cypripedium) orchid medium, contains 400 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 400 mg/L calcium nitrate, without casein. Mother flasking medium V.
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DMEM Low Glucose with Stable Glutamine with Sodium Pyruvate
Supplier: BIOWEST
Dulbecco's Modified Eagle medium (DMEM) is formulated to preserve and maintain the growth of a wide range of mammalian cells. Supplemented with fetal calf serum, DMEM also allows the proliferation of mouse embryonic stem cells.
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Fibroblast Growth Medium, Basal
Supplier: PROVITRO
Fibroblast Growth Medium is a sterile liquid culture medium designed for cultivating human fibroblasts (HFIB). Provided as a basal medium, it is ideal for culturing Provitro HFIB upon the addition of supplement mix components.
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hMSC Chondrogenesis Induction Medium
Supplier: PROVITRO
The hMSC Chondrogenesis Induction Medium is a sterile liquid culture medium specifically designed for initiating chondrogenic differentiation in human mesenchymal stem cells (hMSCs). This medium is provided as a basal formulation and becomes fully functional for hMSC culture upon the addition of all the required supplement mix components.