Runningbuffert
Elektroforesbuffertarna upprätthåller jämna pH-nivåer under separation av DNA, RNA och andra proteiner. De lågladdade lösningarna tillhandahåller joner som överför den applicerade strömmen och möjliggör för olika molekylstorlekar att färdas med olika hastigheter för analyser. De preparativa substanserna ger ytterligare skydd åt proteiner och förhindrar nedbrytning för garanterad provviabilitet och noggrann testning. Den bästa elektroforesbufferten finns i olika molekylvikter och kan väljas baserat på de specifika makromolekyler som hanteras.
MOPS-buffert
Supplier: VWR Chemicals
MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.
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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer
Supplier: VWR Chemicals
TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.
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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade
Supplier: VWR Chemicals
Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)
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VWR® UltraPure, Tris-glycine buffers, 10X concentrate
Supplier: VWR Chemicals
Running buffers for both 1-D and 2-D protein gel electrophoresis.
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VWR® Buffer, MES-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®
Supplier: Merck
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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G-Biosciences TAE Running Buffer (50X)
Supplier: G-Biosciences
The primary use of TAE Running Buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).
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TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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Buffer, PBS (10X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
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G-Biosciences MOPS Running Buffers (10X)
Supplier: G-Biosciences
A zwitterionic buffer providing consistent buffering capacity in the neutral range, ideal for RNA electrophoresis.
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VWR®, Tris-borate-EDTA (TBE) buffer
Supplier: VWR Chemicals
TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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#: 20864.320
Leverantör: VWR Chemicals
#: 83640.320
Leverantör: VWR Chemicals
#: 31770.294
Leverantör: VWR Chemicals
#: 1.00030.2500
Leverantör: Merck
#: 83645.320
Leverantör: VWR Chemicals