Du sökte efter: Kompetenta celler
En vanlig method för transformation av plasmider i bakterier (E.coli) är användningen av kompetenta celler. Kompetenta celler har förändrade cellväggar som kan ta upp främmande DNA i sina interiörer. Kemiskt kompetenta celler används mest för rutinmässig kloning och görs kompetenta genom kalciumklorid och värmeschock. Elektrokompetenta celler används för svårklonade konstrukt. Faktorer som transformationseffektivitet, genetiska markörer och förpackningsformat kommer att påverka tiden och ansträngning som krävs för ditt projekt, och sålunda för dess framgång. Välj mellan ett antal celltyper och format för att säkerställa optimal funktion.
DH5α Chemically Competent E. coli Cells
Supplier: OriGene
Competent Cells, DH5α chemically competent cells (≥10⁸ CFU/μg of pUC19 DNA)
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SCS110 competent cells
Supplier: AGILENT
SCS110 competent cells for generating unmethylated DNA improve quality and yield of miniprep DNA.
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Protein expression competent cell pack
Supplier: AGILENT
Protein Expression competent cell packs efficiently test multiple strains for expression.
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SURE competent cells
Supplier: AGILENT
SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.
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XL-1 red competent cells
Supplier: AGILENT
XL-1 Red Competent Cells are ideal for introducing random mutations into a cloned gene of interest.
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XL10-Gold ultracompetent cells
Supplier: AGILENT
XL10-Gold ultracompetent cells transform large plasmids and ligated DNA with highest efficiency.
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Competent cells, ArcticExpress
Supplier: AGILENT
Tackle two common bacterial gene expression hurdles: protein insolubility and codon bias.
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SCSI supercompetent cells
Supplier: AGILENT
SCSI supercompetent and DH5α competent cells are classic E. coli strains engineered for competence.
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TG1 electroporation-competent cells
Supplier: AGILENT
Phage display libraries from electroporation-competent cells with high transformation efficiency.
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EletroTen-blue electroporation-competent cells
Supplier: AGILENT
Achieve the highest transformation efficiencies available when cloning large plasmids and ligated DNA.
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BL21 competent cells
Supplier: AGILENT
BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.
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XL1 blue and XL2 blue competent cells
Supplier: AGILENT
XL1 and XL2-Blue Competent Cells are versatile for routine cloning and allow blue-white colour screening for high efficiency cloning of methylated DNA.
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TK competent cells
Supplier: AGILENT
Easily induce and express phosphorylated proteins, which can be used to screen expression libraries, to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target.
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ABLE C competent cells
Supplier: AGILENT
ABLE Competent Cells for toxic clones reduce copy number four to ten fold compared to the XL1-Blue strain.
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BJ5183 Electroporation-competent cells
Supplier: AGILENT
BJ5183 electroporation competent cells are a recombination proficient bacterial strain.
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JM competent cells
Supplier: AGILENT
JM Competent Cells are recombination- and E. coli K12 restriction-proficient.
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Competent cell packs
Supplier: AGILENT
High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements.
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Competent cells, Z-Competent™ E. coli transformation kits
Supplier: G-Biosciences
The Z-Competent™ E. coli transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.
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Competent cells, cloning strains, NovaBlue
Supplier: Merck Millipore (Novagen)
NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and recA and endA mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.
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Competent cells, expression strains, B834
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, Rosetta™
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, BLR
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, Origami™ 2
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, HMS174
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, Tuner™
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, BL21
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.