Kompetenta celler
En vanlig method för transformation av plasmider i bakterier (E.coli) är användningen av kompetenta celler. Kompetenta celler har förändrade cellväggar som kan ta upp främmande DNA i sina interiörer. Kemiskt kompetenta celler används mest för rutinmässig kloning och görs kompetenta genom kalciumklorid och värmeschock. Elektrokompetenta celler används för svårklonade konstrukt. Faktorer som transformationseffektivitet, genetiska markörer och förpackningsformat kommer att påverka tiden och ansträngning som krävs för ditt projekt, och sålunda för dess framgång. Välj mellan ett antal celltyper och format för att säkerställa optimal funktion.
ABLE C competent cells
Supplier: AGILENT
ABLE Competent Cells for toxic clones reduce copy number four to ten fold compared to the XL1-Blue strain.
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Competent cells, Z-Competent™ E. coli transformation kits
Supplier: G-Biosciences
The Z-Competent™ E. coli transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.
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Competent cells, expression strains, BLR
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, Origami™ 2
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, cloning strains, NovaBlue
Supplier: Merck Millipore (Novagen)
NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and recA and endA mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.
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Competent cells, expression strains, B834
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, Rosetta™
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, BL21
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, Tuner™
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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Competent cells, expression strains, HMS174
Supplier: Merck Millipore (Novagen)
A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
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