Supplier: Merck Millipore (Novagen)

KOD HiFi DNA Polymerase is a unique proofreading enzyme, isolated from the extreme thermophile Thermococcus kodakaraensis KOD1, which possesses superior processivity and fidelity that enables faster and more accurate PCR amplification than can be achieved with conventional enzymes.

Supplier: Cytiva

illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

Supplier: FINNZYMES REAGENTS

Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Supplier: FINNZYMES REAGENTS

Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

Supplier: FINNZYMES REAGENTS

Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: FINNZYMES REAGENTS

Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

Supplier: Thermo Fisher Scientific

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli. Native Taq DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Supplier: FINNZYMES REAGENTS

Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.

Supplier: FINNZYMES REAGENTS

Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Supplier: FINNZYMES REAGENTS

Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than Taq.

Supplier: MP Biomedicals

The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide Taq DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Supplier: FINNZYMES REAGENTS

Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of Taq DNA polymerase, and 6-fold lower than that of Pfu DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than Pfu DNA polymerase.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

Supplier: Thermo Fisher Scientific

DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing,
phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.

Supplier: FINNZYMES REAGENTS

Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

Supplier: Thermo Fisher Scientific

DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced Taq polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Supplier: Thermo Fisher Scientific

DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimisation of reaction conditions is not required. The enzyme is inhibited by dUTP but can incorporate modified nucleotides. Applications include routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA, RT-PCR, and generation of PCR products for TA cloning.

Supplier: Thermo Fisher Scientific

DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl₂ and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: EDVOTEK

Each PCR Bead contains Taq DNA polymerase, Taq DNA polymerase buffer, dNTP mixture and MgCl₂. Pack contains sufficient for 25 reactions.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Supplier: EPPENDORF

Eppendorf Fast PCR tube strips made of polyethylene transfer heat faster than tubes made of traditional plastics. The Fast PCR tube strips are particularly suited to scientists using Fast PCR reagent kits, scientists trying to speed up protocols or cyclers with high heating and cooling rates.