"Solid Phase Extraction"
Carrier proteins, Imject™ Blue Carrier™
Supplier: Thermo Fisher Scientific
Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.
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Mass tagging, mass tag labelling reagents, AminoxyTMT™, Pierce™
Supplier: Thermo Fisher Scientific
The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).
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Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel
Supplier: Thermo Fisher Scientific
Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.
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FOCUS™ Protein Alkylation Kit
Supplier: G-Biosciences
The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
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Immobilised Trypsin
Supplier: G-Biosciences
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
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Fraction-FOCUS™
Supplier: G-Biosciences
Fraction-FOCUS™ utilises proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins. Fraction-FOCUS™ is fully compatible with all downstream protein identification techniques.
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Carrier proteins, Imject™ Maleimide-Activated Blue Carrier™
Supplier: Thermo Fisher Scientific
Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.
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Phosphoprotein enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.
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Mitochondria Isolation Kit for Tissue
Supplier: Thermo Fisher Scientific
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
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Detergent sampler Kit, Surfact-Amps™
Supplier: Thermo Fisher Scientific
Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
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Immobilized pepsin, Pierce™
Supplier: Thermo Fisher Scientific
Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
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Ubiquitin enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
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FOCUS™ protein reductant kit
Supplier: G-Biosciences
FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
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FOCUS™ protein reduction-alkylation kit
Supplier: G-Biosciences
The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
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Adenine deaminase
Supplier: G-Biosciences
Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
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