"Solid Phase Extraction"
Pierce™ In-solution Tryptic Digestion and Guanidination Kit
Supplier: Thermo Fisher Scientific
The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
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PopCulture® reagent
Supplier: Merck Millipore (Novagen)
PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.
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In-Gel tryptic digestion kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
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Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™
Supplier: G-Biosciences
UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.
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Albumin/IgG removal kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
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Sample Grinding Kit
Supplier: Cytiva
Sample grinding kit is ready to use for disrupting small tissue and cell samples for protein extraction.
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Tryptic Digest of Bovine Serum Albumin
Supplier: BRUKER DALTRONIK
Tryptic digest of bovine serum albumin, for 5×250 spots
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Detergent solutions, Surfact-Amps™
Supplier: Thermo Fisher Scientific
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
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Glycoprotein Isolation Kit, Cona And WGA, Pierce™
Supplier: Thermo Fisher Scientific
The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
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Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins
Supplier: G-Biosciences
FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
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Micrococcal nuclease solution (≥100 U/µl)
Supplier: Thermo Fisher Scientific
This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
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Capsanthin
Supplier: Abcam
Potent antioxidant. Main carotenoid in paprika. Active in vitro. Achieve your results faster with highly validated, pure and trusted compounds.
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Cellulose Soxhlet extraction thimbles, Whatman™
Supplier: Whatman products (Cytiva)
Cytiva's Whatman cellulose thimbles are manufactured from high-quality alpha cellulose cotton linter for high-performance use in automated Soxhlet extractor systems. Used in air and waste gas analysis.
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E.Z.N.A.® RNALock stabilizer reagent
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation



